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Product Info Summary
| SKU: | M00433-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, ICC, WB |
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Product info
Product Name
Anti-SIRT2/Sirtuin 2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00433-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-SIRT2/Sirtuin 2 Rabbit Monoclonal Antibody catalog # M00433-1. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SIRT2/Sirtuin 2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00433-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
EBA-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human SIRT2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00433-1 is reactive to SIRT2 in Human, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
160-180 kDa
Calculated molecular weight
43182 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00433-1 is guaranteed for Flow Cytometry, IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
IP 1:50
FC 1:30
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of SIRT2 expression in SKBR-3 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For SIRT2 (Source: Uniprot.org, NCBI)
Gene Name
SIRT2
Full Name
NAD-dependent protein deacetylase sirtuin-2
Weight
43182 MW
Superfamily
sirtuin family
Alternative Names
NAD-dependent protein deacetylase sirtuin-2;3.5.1.-;Regulatory protein SIR2 homolog 2;SIR2-like protein 2;SIRT2;SIR2L, SIR2L2; SIRT2 SIR2, SIR2L, SIR2L2 sirtuin 2 NAD-dependent protein deacetylase sirtuin-2|NAD-dependent deacetylase sirtuin-2|SIR2-like protein 2|regulatory protein SIR2 homolog 2|silent information regulator 2|sir2-related protein type 2|sirtuin type 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SIRT2, check out the SIRT2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SIRT2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SIRT2/Sirtuin 2 Rabbit Monoclonal Antibody (M00433-1)
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No publications found for M00433-1
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-SIRT2/Sirtuin 2 Rabbit Monoclonal Antibody
Question
See attached the WB image, lot number and protocol we used for testis using anti-SIRT2/Sirtuin 2 Rabbit Monoclonal antibody M00433-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-05-01
Question
Do you have a BSA free version of anti-SIRT2/Sirtuin 2 Rabbit Monoclonal antibody M00433-1 available?
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-SIRT2/Sirtuin 2 Rabbit Monoclonal antibody M00433-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-03
Question
Our lab were well pleased with the WB result of your anti-SIRT2/Sirtuin 2 Rabbit Monoclonal antibody. However we have seen positive staining in brain isoform 1: cytoplasm using this antibody. Is that expected? Could you tell me where is SIRT2 supposed to be expressed?
T. Li
Verified customer
Asked: 2020-01-07
Answer
According to literature, brain does express SIRT2. Generally SIRT2 expresses in nucleus. cytoplasm, perinuclear region., isoform 1: cytoplasm, isoform 2: cytoplasm, isoform 5: cytoplasm. Regarding which tissues have SIRT2 expression, here are a few articles citing expression in various tissues:
Adrenal gland, Pubmed ID: 10931946
Brain, Pubmed ID: 11226170
Brain, and Lung, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Lung, Pubmed ID: 15489334
Testis, Pubmed ID: 10381378
Boster Scientific Support
Answered: 2020-01-07
Question
I was wanting to use your anti-SIRT2/Sirtuin 2 Rabbit Monoclonal antibody for IF for rat testis on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat testis identification?
Verified Customer
Verified customer
Asked: 2019-07-30
Answer
As indicated on the product datasheet, M00433-1 anti-SIRT2/Sirtuin 2 Rabbit Monoclonal antibody has been tested for IP, IF, WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-30
Question
I see that the anti-SIRT2/Sirtuin 2 Rabbit Monoclonal antibody M00433-1 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-10
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-10
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for testis using anti-SIRT2/Sirtuin 2 Rabbit Monoclonal antibody M00433-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-06
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-06
Question
Does anti-SIRT2/Sirtuin 2 Rabbit Monoclonal antibody M00433-1 work for IF with testis?
Verified Customer
Verified customer
Asked: 2019-05-24
Answer
According to the expression profile of testis, SIRT2 is highly expressed in testis. So, it is likely that anti-SIRT2/Sirtuin 2 Rabbit Monoclonal antibody M00433-1 will work for IF with testis.
Boster Scientific Support
Answered: 2019-05-24
Question
My question regarding product M00433-1, anti-SIRT2/Sirtuin 2 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
B. Wu
Verified customer
Asked: 2019-05-03
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00433-1 anti-SIRT2/Sirtuin 2 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-03
Question
Does M00433-1 anti-SIRT2/Sirtuin 2 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-02-01
Answer
As indicated on the product datasheet, M00433-1 anti-SIRT2/Sirtuin 2 Rabbit Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-02-01
Question
Is this M00433-1 anti-SIRT2/Sirtuin 2 Rabbit Monoclonal antibody reactive to the isotypes of SIRT2?
Verified Customer
Verified customer
Asked: 2018-07-06
Answer
The immunogen of M00433-1 anti-SIRT2/Sirtuin 2 Rabbit Monoclonal antibody is A synthesized peptide derived from human SIRT2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-07-06
Question
Will anti-SIRT2/Sirtuin 2 Rabbit Monoclonal antibody M00433-1 work on feline WB with brain?
Verified Customer
Verified customer
Asked: 2018-06-20
Answer
Our lab technicians have not tested anti-SIRT2/Sirtuin 2 Rabbit Monoclonal antibody M00433-1 on feline. You can run a BLAST between feline and the immunogen sequence of anti-SIRT2/Sirtuin 2 Rabbit Monoclonal antibody M00433-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-06-20
Question
I am interested in to test anti-SIRT2/Sirtuin 2 Rabbit Monoclonal antibody M00433-1 on rat testis for research purposes, then I may be interested in using anti-SIRT2/Sirtuin 2 Rabbit Monoclonal antibody M00433-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-03-08
Answer
The products we sell, including anti-SIRT2/Sirtuin 2 Rabbit Monoclonal antibody M00433-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-03-08
Question
Is a blocking peptide available for product anti-SIRT2/Sirtuin 2 Rabbit Monoclonal antibody (M00433-1)?
Verified Customer
Verified customer
Asked: 2018-02-14
Answer
We do provide the blocking peptide for product anti-SIRT2/Sirtuin 2 Rabbit Monoclonal antibody (M00433-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-02-14
Question
We have been able to see staining in human brain lung. What should we do? Is anti-SIRT2/Sirtuin 2 Rabbit Monoclonal antibody supposed to stain brain lung positively?
Verified Customer
Verified customer
Asked: 2017-09-08
Answer
From literature brain lung does express SIRT2. From Uniprot.org, SIRT2 is expressed in c1 segment of cervical spinal cord, testis, adrenal gland, brain lung, lung, brain, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have SIRT2 expression, here are a few articles citing expression in various tissues:
Adrenal gland, Pubmed ID: 10931946
Brain, Pubmed ID: 11226170
Brain, and Lung, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Lung, Pubmed ID: 15489334
Testis, Pubmed ID: 10381378
Boster Scientific Support
Answered: 2017-09-08
Question
We are currently using anti-SIRT2/Sirtuin 2 Rabbit Monoclonal antibody M00433-1 for rat tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human, rat. Is it true that the antibody can work on feline tissues as well?
V. Jones
Verified customer
Asked: 2015-03-30
Answer
The anti-SIRT2/Sirtuin 2 Rabbit Monoclonal antibody (M00433-1) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-03-30
Question
We have tried in the past anti-SIRT2/Sirtuin 2 Rabbit Monoclonal antibody for WB on liver in a previous project. I am using human, and We want to use the antibody for IP next. We are interested in examining liver as well as brain lung in our next experiment. Could you please give me some suggestion on which antibody would work the best for IP?
C. Jha
Verified customer
Asked: 2014-01-14
Answer
I took a look at the website and datasheets of our anti-SIRT2/Sirtuin 2 Rabbit Monoclonal antibody and it appears that M00433-1 has been validated on human in both WB and IP. Thus M00433-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2014-01-14
