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Product Info Summary
| SKU: | PB9160 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-SIRT2 Antibody Picoband®
SKU/Catalog Number
PB9160
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SIRT2 Antibody Picoband® catalog # PB9160. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SIRT2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9160)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human SIRT2, different from the related mouse and rat sequences by three amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9160 is reactive to SIRT2 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
36 kDa
Calculated molecular weight
43182 MW
Background of SIRT2
SIRT2 is a predominantly cytoplasmic protein that colocalizes with microtubules. This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. SIRT2 is mapped to 19q13.2. It is able to cause radioactivity to be transferred from (32P) NAD to bovine serum albumin (BSA). According to researches, SIRT2 can act as a bona fide tubulin deacetylase. SIRT2 was found to deacetylate lys40 of alpha-tubulin both in vitro and in vivo. SIRT2 is also a regulator of mitotic progression that acts downstream from CDC14B in a pathway regulating mitotic exit or subsequent cytokinesis. What’s more, SIRT2 is an important regulator of programmed necrosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9160 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: rat spleen tissue, human Hela whole cell, human A549 whole cell
IHC: mouse brain tissue, rat brain tissue, human lung cancer tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SIRT2 using anti-SIRT2 antibody (PB9160).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIRT2 antigen affinity purified polyclonal antibody (Catalog # PB9160) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP Conjugated secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SIRT2 approximately 36 kDa. The expected band size for SIRT2 is at 43, 40, 41, 30, 36 kDa.
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Figure 2. IHC analysis of SIRT2 using anti-SIRT2 antibody (PB9160).
SIRT2 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-SIRT2 Antibody (PB9160) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IHC analysis of SIRT2 using anti-SIRT2 antibody (PB9160).
SIRT2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-SIRT2 Antibody (PB9160) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of SIRT2 using anti-SIRT2 antibody (PB9160).
SIRT2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-SIRT2 Antibody (PB9160) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For SIRT2 (Source: Uniprot.org, NCBI)
Gene Name
SIRT2
Full Name
NAD-dependent protein deacetylase sirtuin-2
Weight
43182 MW
Superfamily
sirtuin family
Alternative Names
NAD-dependent protein deacetylase sirtuin-2;3.5.1.-;Regulatory protein SIR2 homolog 2;SIR2-like protein 2;SIRT2;SIR2L, SIR2L2; SIRT2 SIR2, SIR2L, SIR2L2 sirtuin 2 NAD-dependent protein deacetylase sirtuin-2|NAD-dependent deacetylase sirtuin-2|SIR2-like protein 2|regulatory protein SIR2 homolog 2|silent information regulator 2|sir2-related protein type 2|sirtuin type 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SIRT2, check out the SIRT2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SIRT2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SIRT2 Antibody Picoband® (PB9160)
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Customer Q&As
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11 Customer Q&As for Anti-SIRT2 Antibody Picoband®
Question
I am looking for using your anti-SIRT2 antibody for myelination in peripheral nervous system studies. Has this antibody been tested with western blotting on mouse brain? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-04-03
Answer
We appreciate your inquiry. This PB9160 anti-SIRT2 antibody is validated on rat spleen tissue, tissue lysate, hela whole cell lysate, a549 whole cell lysate, mouse brain, brain tissue, lung cancer tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-04-03
Question
My lab would like to test anti-SIRT2 antibody PB9160 on human brain lung for research purposes, then I may be interested in using anti-SIRT2 antibody PB9160 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-16
Answer
The products we sell, including anti-SIRT2 antibody PB9160, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-16
Question
Please see the WB image, lot number and protocol we used for brain lung using anti-SIRT2 antibody PB9160. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-29
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-29
Question
We are currently using anti-SIRT2 antibody PB9160 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-25
Answer
The anti-SIRT2 antibody (PB9160) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-25
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain lung using anti-SIRT2 antibody PB9160. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-17
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-17
Question
I was wanting to use your anti-SIRT2 antibody for WB for human brain lung on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human brain lung identification?
Verified Customer
Verified customer
Asked: 2018-11-05
Answer
You can see on the product datasheet, PB9160 anti-SIRT2 antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human brain lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-11-05
Question
Does anti-SIRT2 antibody PB9160 work for WB with brain lung?
R. Kulkarni
Verified customer
Asked: 2018-09-17
Answer
According to the expression profile of brain lung, SIRT2 is highly expressed in brain lung. So, it is likely that anti-SIRT2 antibody PB9160 will work for WB with brain lung.
Boster Scientific Support
Answered: 2018-09-17
Question
Our team were happy with the WB result of your anti-SIRT2 antibody. However we have observed positive staining in adrenal gland perinuclear region. using this antibody. Is that expected? Could you tell me where is SIRT2 supposed to be expressed?
F. Miller
Verified customer
Asked: 2016-12-06
Answer
According to literature, adrenal gland does express SIRT2. Generally SIRT2 expresses in nucleus. cytoplasm, perinuclear region., isoform 1: cytoplasm, isoform 2: cytoplasm, isoform 5: cytoplasm. Regarding which tissues have SIRT2 expression, here are a few articles citing expression in various tissues:
Adrenal gland, Pubmed ID: 10931946
Brain, Pubmed ID: 11226170
Brain, and Lung, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Lung, Pubmed ID: 15489334
Testis, Pubmed ID: 10381378
Boster Scientific Support
Answered: 2016-12-06
Question
Is this PB9160 anti-SIRT2 antibody reactive to the isotypes of SIRT2?
K. Jha
Verified customer
Asked: 2016-11-23
Answer
The immunogen of PB9160 anti-SIRT2 antibody is A synthetic peptide corresponding to a sequence in the middle region of human SIRT2(165-187aa YTQNIDTLERIAGLEQEDLVEAH), different from the related mouse and rat sequences by three amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-11-23
Question
We have observed staining in human leukemic t-cell. Do you have any suggestions? Is anti-SIRT2 antibody supposed to stain leukemic t-cell positively?
C. Anderson
Verified customer
Asked: 2016-06-14
Answer
Based on literature leukemic t-cell does express SIRT2. Based on Uniprot.org, SIRT2 is expressed in c1 segment of cervical spinal cord, testis, adrenal gland, brain lung, lung, brain, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have SIRT2 expression, here are a few articles citing expression in various tissues:
Adrenal gland, Pubmed ID: 10931946
Brain, Pubmed ID: 11226170
Brain, and Lung, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Lung, Pubmed ID: 15489334
Testis, Pubmed ID: 10381378
Boster Scientific Support
Answered: 2016-06-14
Question
We ordered your anti-SIRT2 antibody for IHC on brain lung in a previous project. I am using rat, and We are going to use the antibody for WB next. I am looking for examining brain lung as well as brain in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
E. Zhao
Verified customer
Asked: 2015-06-12
Answer
I have checked the website and datasheets of our anti-SIRT2 antibody and it seems that PB9160 has been tested on rat in both IHC and WB. Thus PB9160 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2015-06-12
