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Product Info Summary
| SKU: | PA2113 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-SIDT1 Antibody
SKU/Catalog Number
PA2113
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SIDT1 Antibody catalog # PA2113. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SIDT1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2113)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human SIDT1, different from the related rat sequence by five amino acids, and different from the related mouse sequence by three amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2113 is reactive to SIDT1 in Human
Applications
PA2113 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
44 kDa
Calculated molecular weight
93839 MW
Background of SIDT1
SID1 transmembrane family member 1, also called SID1, C. ELEGANS, is a protein which in humans is encoded by the SIDT1 gene. The protein encoded by this gene links to HGNC-curated gene family pages. The gene was assigned to human chromosome 3q13.31. This protein likely plays an important role in RNA transmembrane transporter activity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Anti-SIDT1 antibody, PA2113, Western blotting
Lane 1: HELA Cell Lysate
Lane 2: COLO320 Cell Lysate
Lane 3: SW620 Cell Lysate
Lane 4: HT1080 Cell Lysate
Protein Target Info & Infographic
Gene/Protein Information For SIDT1 (Source: Uniprot.org, NCBI)
Gene Name
SIDT1
Full Name
SID1 transmembrane family member 1
Weight
93839 MW
Superfamily
SID1 family
Alternative Names
B830021E24Rik; FLJ20174; SID1 transmembrane family member 1; SID1 transmembrane family, member 1; SID1; SID-1 SIDT1 SID-1, SID1 SID1 transmembrane family member 1 SID1 transmembrane family member 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SIDT1, check out the SIDT1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SIDT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SIDT1 Antibody (PA2113)
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No publications found for PA2113
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-SIDT1 Antibody
Question
I am interested in to test anti-SIDT1 antibody PA2113 on human right hemisphere of cerebellum for research purposes, then I may be interested in using anti-SIDT1 antibody PA2113 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-02
Answer
The products we sell, including anti-SIDT1 antibody PA2113, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-02
Question
We are currently using anti-SIDT1 antibody PA2113 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-04-26
Answer
The anti-SIDT1 antibody (PA2113) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-04-26
Question
Does anti-SIDT1 antibody PA2113 work on feline WB with right hemisphere of cerebellum?
P. Gonzalez
Verified customer
Asked: 2018-11-19
Answer
Our lab technicians have not validated anti-SIDT1 antibody PA2113 on feline. You can run a BLAST between feline and the immunogen sequence of anti-SIDT1 antibody PA2113 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline right hemisphere of cerebellum in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-11-19
Question
Does PA2113 anti-SIDT1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Z. Lewis
Verified customer
Asked: 2018-10-31
Answer
It shows on the product datasheet, PA2113 anti-SIDT1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-10-31
Question
Here is the WB image, lot number and protocol we used for right hemisphere of cerebellum using anti-SIDT1 antibody PA2113. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-10-25
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-10-25
Question
Is this PA2113 anti-SIDT1 antibody reactive to the isotypes of SIDT1?
Verified Customer
Verified customer
Asked: 2017-08-16
Answer
The immunogen of PA2113 anti-SIDT1 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human SIDT1(36-52aa RRDPFDAARGADFDHVY), different from the related rat sequence by five amino acids, and different from the related mouse sequence by three amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-08-16
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for right hemisphere of cerebellum using anti-SIDT1 antibody PA2113. Let me know if you need anything else.
R. Lewis
Verified customer
Asked: 2016-07-19
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-07-19
