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Product Info Summary
| SKU: | A00938 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-SHP-1 (Ab-536) PTPN6 Antibody
SKU/Catalog Number
A00938
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-SHP-1 (Ab-536) PTPN6 Antibody (Catalog # A00938). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SHP-1 (Ab-536) PTPN6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00938)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide sequence around aa.534~538 (S-E-Y-G-N) derived from Human SHP-1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00938 is reactive to PTPN6 in Human, Mouse
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
34006 MW
Background of SHP-1
Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus.
Migone TS, et al. (1998) Proc Natl Acad Sci USA; 95(7): 3845-3850.
Timms JF, et al.(1998) Mol Cell Biol; 18(7): 3838-3850.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00938 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 68kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Validation Images & Assay Conditions
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Figure 1. IHC analysis of PTPN6 using anti-PTPN6 antibody (A00938).
PTPN6 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PTPN6 Antibody (A00938) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Immunofluorescence staining of methanol-fixed MCF cells using SHP-1(Ab-536) Antibody #A00938.
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Figure 3. Western blot analysis of PTPN6 using anti-PTPN6 antibody (A00938).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTPN6 antigen affinity purified polyclonal antibody (Catalog # A00938) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for PTPN6.
Protein Target Info & Infographic
Gene/Protein Information For PTPN6 (Source: Uniprot.org, NCBI)
Gene Name
PTPN6
Full Name
Tyrosine-protein phosphatase non-receptor type 6
Weight
34006 MW
Superfamily
protein-tyrosine phosphatase family
Alternative Names
Glycerol-3-phosphate phosphatase ;G3PP ;3.1.3.21 ;Aspartate-based ubiquitous Mg (2+)-dependent phosphatase ;AUM ;3.1.3.48 ;Phosphoglycolate phosphatase ;PGP ;PGP ; PTPN6 HCP, HCPH, HPTP1C, PTP-1C, SH-PTP1, SHP-1, SHP-1L, SHP1 protein tyrosine phosphatase non-receptor type 6 tyrosine-protein phosphatase non-receptor type 6|hematopoietic cell phosphatase|hematopoietic cell protein-tyrosine phosphatase|protein-tyrosine phosphatase 1C|protein-tyrosine phosphatase SHP-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PTPN6, check out the PTPN6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PTPN6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SHP-1 (Ab-536) PTPN6 Antibody (A00938)
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