Click image to see more details
Product Info Summary
| SKU: | A30456 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB, IF (paraffin) |
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Product info
Product Name
Anti-SGOL1 SGO1 Antibody
SKU/Catalog Number
A30456
Size
100ul
Form
Liquid
Description
Boster Bio Anti-SGOL1 SGO1 Antibody (Catalog# A30456). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Rat, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SGOL1 SGO1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30456)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human SGOL1. Immunogen sequence location: 271-320
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A30456 is reactive to Sgo1 in Human, Mouse, Rat
Applications
A30456 is guaranteed for ELISA, IHC-P, WB, IF (paraffin) Boster Guarantee
Observed Molecular Weight
72 kDa
Calculated molecular weight
64190 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:20000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western blot analysis of lysates from HeLa cells, using SGOL1 Antibody. The lane on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For Sgo1 (Source: Uniprot.org, NCBI)
Gene Name
Sgo1
Full Name
Shugoshin 1
Weight
64190 MW
Superfamily
shugoshin family
Alternative Names
FLJ14230; hSgo1; NY-BR-85; Serologically defined breast cancer antigen NY-BR-85; SGO; Sgo1; shugoshin 1AB protein; shugoshin 1CD protein; shugoshin 1EF protein; shugoshin 1GH protein; shugoshin 1KL protein; shugoshin-like 1 (S. pombe); shugoshin-like 1 Sgo1|3300001M08Rik, C81037, Sg, Sgol1|shugoshin 1|shugoshin 1|shugoshin 1A protein|shugoshin-like 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Sgo1, check out the Sgo1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Sgo1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SGOL1 SGO1 Antibody (A30456)
Hello CJ!
No publications found for A30456
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-SGOL1 SGO1 Antibody
Question
We are currently using anti-SGOL1 antibody A30456 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-03
Answer
The anti-SGOL1 antibody (A30456) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-03
Question
Can you help my question with product A30456, anti-SGOL1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-08-16
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30456 anti-SGOL1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-08-16
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-SGOL1 antibody A30456. Let me know if you need anything else.
R. Carter
Verified customer
Asked: 2018-07-02
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-07-02
Question
Is a blocking peptide available for product anti-SGOL1 antibody (A30456)?
Verified Customer
Verified customer
Asked: 2018-03-30
Answer
We do provide the blocking peptide for product anti-SGOL1 antibody (A30456). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-03-30
Question
I was wanting to use to test anti-SGOL1 antibody A30456 on human cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-SGOL1 antibody A30456 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
C. Moore
Verified customer
Asked: 2013-11-15
Answer
The products we sell, including anti-SGOL1 antibody A30456, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-11-15
