Product Info Summary
SKU: | A03748-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IHC, IHC-F, ICC, WB |
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Product info
Product Name
Anti-SGLT2/SLC5A2 Antibody Picoband®
View all SGLT2/SLC5A2 Antibodies
SKU/Catalog Number
A03748-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SGLT2/SLC5A2 Antibody Picoband® catalog # A03748-1. Tested in ELISA, Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SGLT2/SLC5A2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03748-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Standard Protein
E.coli-derived human SGLT2/SLC5A2 recombinant protein (Position: A15-N656).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03748-1 is reactive to SLC5A2 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
73 kDa
Background of SGLT2/SLC5A2
The sodium/glucose cotransporter 2 (SGLT2) is a protein that in humans is encoded by the SLC5A2 gene. It is mapped to 16p11.2. This gene encodes a member of the sodium glucose cotransporter family which are sodium-dependent glucose transport proteins. The encoded protein is the major cotransporter involved in glucose reabsorption in the kidney. Mutations in this gene are associated with renal glucosuria. Two transcript variants, one protein-coding and one not, have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03748-1 is guaranteed for ELISA, Flow Cytometry, IHC, IHC-F, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunohistochemistry (Frozen Section), 0.5-1μg/ml
Immunocytochemistry, 0.5-1μg/ml
Flow Cytometry (Fixed), 1-3μg/1x106 cells
Direct ELISA, 0.1-0.5μg/ml
Positive Control
WB: human HL-60 whole cell, human THP-1 whole cell, rat kidney tissue, rat spleen tissue, rat lung tissue, mouse kidney tissue, mouse spleen tissue, mouse lung tissue,, human HL-60 whole cell, human THP-1 whole cell, rat kidney tissue, rat spleen tissue, rat lung tissue, mouse kidney tissue, mouse spleen tissue, mouse lung tissue
IHC: human renal cancer tissue, mouse kidney tissue, rat kidney tissue
FCM: HepG2 cell
Validation Images & Assay Conditions
![a03748 1 slc5a2 primary antibodies wb testing 1 a03748 1 slc5a2 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a03748-1-slc5a2-primary-antibodies-wb-testing-1.jpg)
Click image to see more details
Figure 1. Western blot analysis of SLC5A2 using anti-SLC5A2 antibody (A03748-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HL-60 whole cell lysates,
Lane 2: human THP-1 whole cell lysates,
Lane 3: rat kidney tissue lysates,
Lane 4: rat spleen tissue lysates,
Lane 5: rat lung tissue lysates,
Lane 6: mouse kidney tissue lysates,
Lane 7: mouse spleen tissue lysates,
Lane 8: mouse lung tissue lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC5A2 antigen affinity purified polyclonal antibody (Catalog # A03748-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC5A2 at approximately 73KD. The expected band size for SLC5A2 is at 73KD.
![a03748 1 slc5a2 primary antibodies ihc testing 2 a03748 1 slc5a2 primary antibodies ihc testing 2](https://www.bosterbio.com/media/catalog/product/a/0/a03748-1-slc5a2-primary-antibodies-ihc-testing-2.jpg)
Click image to see more details
Figure 2. IHC analysis of SLC5A2 using anti-SLC5A2 antibody (A03748-1).
SLC5A2 was detected in paraffin-embedded section of human renal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC5A2 Antibody (A03748-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
![a03748 1 slc5a2 primary antibodies ihc testing 3 a03748 1 slc5a2 primary antibodies ihc testing 3](https://www.bosterbio.com/media/catalog/product/a/0/a03748-1-slc5a2-primary-antibodies-ihc-testing-3.jpg)
Click image to see more details
Figure 3. IHC analysis of SLC5A2 using anti-SLC5A2 antibody (A03748-1).
SLC5A2 was detected in paraffin-embedded section of mouse kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC5A2 Antibody (A03748-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
![A03748 1 SLC5A2 primary antibodies IHC testing 3 A03748 1 SLC5A2 primary antibodies IHC testing 3](https://www.bosterbio.com/media/catalog/product/A/0/A03748-1-SLC5A2-primary-antibodies-IHC-testing-3.jpg)
Click image to see more details
Figure 3. IHC analysis of SLC5A2 using anti-SLC5A2 antibody (A03748-1).
SLC5A2 was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SLC5A2 Antibody (A03748-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
![a03748 1 slc5a2 primary antibodies fc testing 5 a03748 1 slc5a2 primary antibodies fc testing 5](https://www.bosterbio.com/media/catalog/product/a/0/a03748-1-slc5a2-primary-antibodies-fc-testing-5.png)
Click image to see more details
Figure 5. Flow Cytometry analysis of HepG2 cells using anti-SLC5A2 antibody (A03748-1).
Overlay histogram showing HepG2 cells stained with A03748-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC5A2 Antibody (A03748-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
![A03748 1 SLC5A2 primary antibodies WB testing 6 A03748 1 SLC5A2 primary antibodies WB testing 6](https://www.bosterbio.com/media/catalog/product/A/0/A03748-1-SLC5A2-primary-antibodies-WB-testing-6.jpg)
Click image to see more details
Figure 6. Western blot analysis of SLC5A2 using anti-SLC5A2 antibody (A03748-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HL-60 whole cell lysates,
Lane 2: human THP-1 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC5A2 antigen affinity purified polyclonal antibody (Catalog # A03748-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC5A2 at approximately 73KD. The expected band size for SLC5A2 is at 73KD.
![A03748 1 SLC5A2 primary antibodies WB testing 7 A03748 1 SLC5A2 primary antibodies WB testing 7](https://www.bosterbio.com/media/catalog/product/A/0/A03748-1-SLC5A2-primary-antibodies-WB-testing-7.jpg)
Click image to see more details
Figure 7. Western blot analysis of SLC5A2 using anti-SLC5A2 antibody (A03748-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat kidney tissue lysates,
Lane 2: rat spleen tissue lysates,
Lane 3: rat lung tissue lysates,
Lane 4: mouse kidney tissue lysates,
Lane 5: mouse spleen tissue lysates,
Lane 6: mouse lung tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC5A2 antigen affinity purified polyclonal antibody (Catalog # A03748-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC5A2 at approximately 73KD. The expected band size for SLC5A2 is at 73KD.
Protein Target Info & Infographic
Gene/Protein Information For SLC5A2 (Source: Uniprot.org, NCBI)
Gene Name
SLC5A2
Full Name
Sodium/glucose cotransporter 2
Weight
Superfamily
sodium:solute symporter (SSF) (TC 2.A.21) family
Alternative Names
Low affinity sodium-glucose cotransporter; Na(+)/glucose cotransporter 2; SGLT2; SGLT2sodium/glucose cotransporter 2; SLC5A2; solute carrier family 5 (sodium/glucose cotransporter), member 2; solute carrier family 5 (sodium/glucose transporter), member 2; Solute carrier family 5 member 2 SLC5A2 SGLT2 solute carrier family 5 member 2 sodium/glucose cotransporter 2|Na(+)/glucose cotransporter 2|low affinity sodium-glucose cotransporter|solute carrier family 5 (sodium/glucose cotransporter), member 2|solute carrier family 5 (sodium/glucose transporter), member 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SLC5A2, check out the SLC5A2 Infographic
![SLC5A2 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SLC5A2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SGLT2/SLC5A2 Antibody Picoband® (A03748-1)
Hello CJ!
A03748-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
High-Salt Attenuates the Efficacy of Dapagliflozin in Tubular Protection by Impairing Fatty Acid Metabolism in Diabetic Kidney Disease.
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Customer Q&As
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4 Customer Q&As for Anti-SGLT2/SLC5A2 Antibody Picoband®
Question
Do you have a protocol in using the blocking peptide for Western Blot application with A03748-1?
Verified customer
Asked: 2020-11-06
Answer
The antigen of the Anti-SGLT2/SLC5A2 Antibody Picoband™ (A03748-1) is a recombinant protein rather than a peptide. However our lab hasn't performed a WB test using the recombinant protein and we suggest to run a pilot test using the antigen.
Boster Scientific Support
Answered: 2020-11-09
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for adult mammalian kidney using anti-SGLT2/SLC5A2 antibody A03748-1. Let me know if you need anything else.
C. Edwards
Verified customer
Asked: 2019-02-26
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-02-26
Question
We are currently using anti-SGLT2/SLC5A2 antibody A03748-1 for rat tissue, and we are satisfied with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-01-31
Answer
The anti-SGLT2/SLC5A2 antibody (A03748-1) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-01-31
Question
I have a question about product A03748-1, anti-SGLT2/SLC5A2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
R. Banerjee
Verified customer
Asked: 2017-10-26
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03748-1 anti-SGLT2/SLC5A2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-10-26