Product Info Summary
SKU: | A06989 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-SGK269 PEAK1 Antibody
SKU/Catalog Number
A06989
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-SGK269 PEAK1 Antibody (Catalog # A06989). Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SGK269 PEAK1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06989)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from N-terminal of human SGK269.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06989 is reactive to PEAK1 in Human, Mouse
Applications
A06989 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
193106 MW
Background of PEAK1
Tyrosine kinase that may play a role in cell spreading and migration on fibronectin. May directly or indirectly affect phosphorylation levels of cytoskeleton-associated proteins MAPK1/ERK and PXN.
Zody M.C., Nature 440:671-675(2006).
Jikuya H., Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases.
Ohara O., DNA Res. 9:47-57(2002).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from 3T3 cells, using SGK269 antibody A06989.
Protein Target Info & Infographic
Gene/Protein Information For PEAK1 (Source: Uniprot.org, NCBI)
Gene Name
PEAK1
Full Name
Inactive tyrosine-protein kinase PEAK1
Weight
193106 MW
Superfamily
protein kinase superfamily
Alternative Names
FLJ21140; FLJ34483; KIAA2002; NKF3 kinase family member; pseudopodium enriched atypical kinase; pseudopodium-enriched atypical kinase 1; SGK269; sugen kinase 269; tyrosine-protein kinase SgK269 PEAK1 SGK269 pseudopodium enriched atypical kinase 1 inactive tyrosine-protein kinase PEAK1|NKF3 kinase family member|sugen kinase 269|tyrosine-protein kinase SgK269
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PEAK1, check out the PEAK1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PEAK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SGK269 PEAK1 Antibody (A06989)
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No publications found for A06989
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-SGK269 PEAK1 Antibody
Question
We are currently using anti-SGK269 antibody A06989 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-08
Answer
The anti-SGK269 antibody (A06989) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-08
Question
See below the WB image, lot number and protocol we used for cervix carcinoma using anti-SGK269 antibody A06989. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-05-23
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-23
Question
My question regarding product A06989, anti-SGK269 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-03-21
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A06989 anti-SGK269 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-03-21
Question
Do you have a BSA free version of anti-SGK269 antibody A06989 available?
Verified Customer
Verified customer
Asked: 2018-03-22
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-SGK269 antibody A06989 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-03-22
Question
Will anti-SGK269 antibody A06989 work for WB with cervix carcinoma?
S. Yang
Verified customer
Asked: 2018-01-29
Answer
According to the expression profile of cervix carcinoma, PEAK1 is highly expressed in cervix carcinoma. So, it is likely that anti-SGK269 antibody A06989 will work for WB with cervix carcinoma.
Boster Scientific Support
Answered: 2018-01-29
Question
Will anti-SGK269 antibody A06989 work on horse WB with mesangial cell smooth muscle?
Verified Customer
Verified customer
Asked: 2017-10-30
Answer
Our lab technicians have not validated anti-SGK269 antibody A06989 on horse. You can run a BLAST between horse and the immunogen sequence of anti-SGK269 antibody A06989 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse mesangial cell smooth muscle in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-10-30
Question
I was wanting to use your anti-SGK269 antibody for WB for mouse cervix carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse cervix carcinoma identification?
G. Baker
Verified customer
Asked: 2015-03-27
Answer
It shows on the product datasheet, A06989 anti-SGK269 antibody has been validated for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-03-27