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Product Info Summary
| SKU: | PB9362 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-SFRP2 Antibody Picoband®
SKU/Catalog Number
PB9362
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SFRP2 Antibody Picoband® catalog # PB9362. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SFRP2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9362)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SFRP2 recombinant protein (Position: L104-C295). Human SFRP2 shares 99% amino acid (aa) sequence identity with mouse SFRP2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9362 is reactive to SFRP2 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
33 kDa
Calculated molecular weight
33490 MW
Background of sFRP-2
SFRP2 is also known as FRP-2, SARP1 or SDF-5. It is Expressed in adipose tissue, heart, brain, skeletal muscle, pancreas, thymus, prostate, testis, ovary, small intestine and colon. Highest levels in adipose tissue, small intestine and colon. This gene encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. Methylation of this gene is a potential marker for the presence of colorectal cancer.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9362 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, -
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: COLO320 Whole Cell, SW620 Whole Cell
Validation Images & Assay Conditions
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Anti-SFRP2 Picoband antibody, PB9362, Western blotting
All lanes: Anti SFRP2 (PB9362) at 0.5ug/ml
Lane 1: COLO320 Whole Cell Lysate at 40ug
Lane 2: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 33KD
Observed bind size: 33KD
Protein Target Info & Infographic
Gene/Protein Information For SFRP2 (Source: Uniprot.org, NCBI)
Gene Name
SFRP2
Full Name
Secreted frizzled-related protein 2
Weight
33490 MW
Superfamily
secreted frizzled-related protein (sFRP) family
Alternative Names
Secreted frizzled-related protein 2;FRP-2;sFRP-2;Secreted apoptosis-related protein 1;SARP-1;SFRP2;FRP2, SARP1;FKSG12, UNQ361/PRO697; SFRP2 FRP-2, SARP1, SDF-5 secreted frizzled related protein 2 secreted frizzled-related protein 2|SARP-1|sFRP-2|secreted apoptosis related protein 1|testicular tissue protein Li 170
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SFRP2, check out the SFRP2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SFRP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SFRP2 Antibody Picoband® (PB9362)
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Customer Q&As
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4 Customer Q&As for Anti-SFRP2 Antibody Picoband®
Question
We are currently using anti-SFRP2 antibody PB9362 for human tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-18
Answer
The anti-SFRP2 antibody (PB9362) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-18
Question
I have a question about product PB9362, anti-SFRP2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-04
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9362 anti-SFRP2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-04
Question
I see that the anti-SFRP2 antibody PB9362 works with ELISA, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-01
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-08-01
Question
Does anti-SFRP2 antibody PB9362 work on bovine WB with upper arm skin?
M. Lewis
Verified customer
Asked: 2016-01-05
Answer
Our lab technicians have not tested anti-SFRP2 antibody PB9362 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-SFRP2 antibody PB9362 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine upper arm skin in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-01-05
