Click image to see more details
Product Info Summary
| SKU: | M05349-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IF, IHC-P, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-SET07 Antibody
SKU/Catalog Number
M05349-1
Size
80 µl
Description
Boster Bio Anti-SET07 Antibody (Catalog # M05349-1). Tested in WB, IF, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-SET07 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05349-1)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
43AT551.86
Isotype
Mouse IgG1
Immunogen
This SET07 antibody was raised using purified recombinant GST fusion protein encoding the N-terminal region of human SET07.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M05349-1 is reactive to Kmt5a in Human
Applications
M05349-1 is guaranteed for IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
42890 Da
Background of SETD8
Protease that can remove conjugated ubiquitin from target proteins and polyubiquitin chains. Inhibits the degradation of target proteins by the proteasome. Plays a role in the regulation of pathways leading to NF-kappa-B activation. Plays a role in the regulation of DNA repair after double-stranded DNA breaks.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IF: 1:10-1:50
IHC-P: 1:10-1:50
Validation Images & Assay Conditions
Click image to see more details
All lanes :SET07 Antibody at 1:2000 dilution
Lane 1: 293T whole cell lysate
Lane 2: 293 whole cell lysate
Lane 3: K562 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 43 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Click image to see more details
(TOP)Negative control of hela cells without PE-conjugated goat anti-mouse lgG (whole molecule).PE-conjugated goat anti-mouse lgG emits red fluorescence. ( Bottom)Immunofluorescence analysis of SET07 Antibody in HeLa cells. 0.025 mg/ml primary antibody was followed by PE-conjugated goat anti-mouse lgG (whole molecule).
Click image to see more details
Formalin-fixed and paraffin-embedded human testis tissue reacted with SET07 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Protein Target Info & Infographic
Gene/Protein Information For Kmt5a (Source: Uniprot.org, NCBI)
Gene Name
Kmt5a
Full Name
N-lysine methyltransferase KMT5A
Weight
42890 Da
Superfamily
class V-like SAM-binding methyltransferase superfamily
Alternative Names
H4-K20-HMTase SETD8; H4K20-specific histone methyltransferase splice variant Set8b; Histone-lysine N-methyltransferase SETD8; KMT5A SET domain-containing protein 8; KMT5A; Lysine N-methyltransferase 5A; PR/SET domain containing protein 8; PR/SET domain-containing protein 07; PR/SET07; PRSET7; PR-Set7EC 2.1.1.43; SET domain containing (lysine methyltransferase) 8; SET07SET8H4-K20-specific histone methyltransferase; SET8 Kmt5a|2410195B05Rik, AA617402, AW536475, PR-S, PR-SET7, PR/SET07, Setd, Setd8|lysine methyltransferase 5A|N-lysine methyltransferase KMT5A|H4-K20-HMTase KMT5A|H4-K20-HMTase SETD8|N-lysine methyltransferase SETD8|PR/SET domain-containing protein 07|SET domain containing (lysine methyltransferase) 8|SET domain-containing protein 8|histone-lysine N-methyltransferase KMT5A|histone-lysine N-methyltransferase SETD8|lysine-specific methylase 5A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Kmt5a, check out the Kmt5a Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Kmt5a: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SET07 Antibody (M05349-1)
Hello CJ!
No publications found for M05349-1
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-SET07 Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-SET07 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
