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Product Info Summary
| SKU: | RP1054 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-SERCA2 ATPase/ATP2A2 Antibody
View all SERCA1 ATPase Antibodies
SKU/Catalog Number
RP1054
PB0279 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SERCA2 ATPase/ATP2A2 Antibody catalog # RP1054. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SERCA2 ATPase/ATP2A2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1054)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human SERCA2 ATPase, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1054 is reactive to ATP2A1 in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
115 kDa
Calculated molecular weight
114757 MW
Background of SERCA1 ATPase
SERCA2, also called ATP2A2 or ATP2B, encodes one of the SERCA Ca (2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. They are closely related to the plasma membrane Ca (2+)-ATPases, or PMCAs. SERCA2 belongs to the large family of P-type cation pumps that couple ATP hydrolysis with cation transport across membranes. The SERCA2 gene is mapped to 12q24.11. SERCA2 was expressed in all specimens, with pronounced expression in the subnuclear aspect of basal epidermal keratinocytes. There was variable suprabasal expression. SERCA2 expression was also observed in the infundibulum and outer root sheath of hair follicles; germinative and mature cells of sebaceous glands; secretory coil and duct of eccrine glands; apocrine gland cells; and arrector pili muscle. In Darier disease skin, strong SERCA2 positivity was detected in the basal, suprabasal, and acantholytic lesional cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
RP1054 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat
Positive Control
WB: rat heart tissue, mouse heart tissue
IHC: Mouse Lung tissue, Rat Lung tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ATP2A2 using anti-ATP2A2 antibody (RP1054).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP2A2 antigen affinity purified polyclonal antibody (Catalog # RP1054) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP2A2 at approximately 115 kDa. The expected band size for ATP2A2 is at 115 kDa.
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Figure 2. IHC analysis of ATP2A2 using anti-ATP2A2 antibody (RP1054).
ATP2A2 was detected in a paraffin-embedded section of Mouse Lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ATP2A2 Antibody (RP1054) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IHC analysis of ATP2A2 using anti-ATP2A2 antibody (RP1054).
ATP2A2 was detected in a paraffin-embedded section of Rat Lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ATP2A2 Antibody (RP1054) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For ATP2A1 (Source: Uniprot.org, NCBI)
Gene Name
ATP2A1
Full Name
Sarcoplasmic/endoplasmic reticulum calcium ATPase 1
Weight
114757 MW
Superfamily
cation transport ATPase (P-type) (TC 3.A.3) family
Alternative Names
Sarcoplasmic/endoplasmic reticulum calcium ATPase 2;SERCA2;SR Ca (2+)-ATPase 2;3.6.3.8;Calcium pump 2;Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform;Endoplasmic reticulum class 1/2 Ca (2+) ATPase;ATP2A2;ATP2B; ATP2A1 ATP2A, SERCA1 ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 1 sarcoplasmic/endoplasmic reticulum calcium ATPase 1|ATPase, Ca++ transporting, cardiac muscle, fast twitch 1|SR Ca(2+)-ATPase 1|calcium pump 1|endoplasmic reticulum class 1/2 Ca(2+) ATPase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATP2A1, check out the ATP2A1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATP2A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SERCA2 ATPase/ATP2A2 Antibody (RP1054)
Hello CJ!
RP1054 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Guo Y, Zeng Qc, Zhang Cq, Zhang Xz, Li Rx, Wu Jm, Guan J, Liu L, Zhang Xc, Li Jy, Wan Zm. Int J Med Sci. 2013 Nov 5;10(13):1837-45. Doi: 10.7150/Ijms.6786. Ecollection 2013. Extracellular Matrix Of Mechanically Stretched Cardiac Fibroblasts Improv...
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