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Product Info Summary
| SKU: | A09239-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-SEMA3D Antibody Picoband®
SKU/Catalog Number
A09239-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-SEMA3D Antibody Picoband® catalog # A09239-1. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-SEMA3D Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09239-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SEMA3D recombinant protein (Position: D88-H697).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A09239-1 is reactive to SEMA3D in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
90 kDa
Calculated molecular weight
128790 MW
Background of Sema3d
This gene encodes a member of the semaphorin III family of secreted signaling proteins that are involved in axon guidance during neuronal development. The encoded protein contains an N-terminal Sema domain, an immunoglobulin like domain and a C-terminal basic domain. The protein encoded by this gene binds neuropilin and plays an important role in cardiovascular development.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09239-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Hacat whole cell, human PC-3 whole cell, human 293T whole cell, human U20S whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SEMA3D using anti-SEMA3D antibody (A09239-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hacat whole cell lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human U20S whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEMA3D antigen affinity purified polyclonal antibody (Catalog # A09239-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEMA3D at approximately 90 kDa. The expected band size for SEMA3D is at 88,90 kDa.
Protein Target Info & Infographic
Gene/Protein Information For SEMA3D (Source: Uniprot.org, NCBI)
Gene Name
SEMA3D
Full Name
Semaphorin-3D
Weight
128790 MW
Superfamily
semaphorin family
Alternative Names
Nck-associated protein 1;NAP 1;Membrane-associated protein HEM-2;p125Nap1;NCKAP1;HEM2, KIAA0587, NAP1; Sema3d|4631426B19Rik|sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D|semaphorin-3D
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SEMA3D, check out the SEMA3D Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SEMA3D: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SEMA3D Antibody Picoband® (A09239-1)
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