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Product Info Summary
| SKU: | PB9679 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-SECTM1/Sectm1b Antibody Picoband®
SKU/Catalog Number
PB9679
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SECTM1/Sectm1b Antibody Picoband® catalog # PB9679. Tested in WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SECTM1/Sectm1b Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9679)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse SECTM1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9679 is reactive to Sectm1b in Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
23 kDa
Calculated molecular weight
23477 MW
Background of Sectm1b
SECTM1B is also known as Sectm1 or K12. Secreted and transmembrane protein 1 is a protein that in humans is encoded by the SECTM1 gene. This gene encodes a transmembrane and secreted protein with characteristics of a type 1a transmembrane protein. It is found in a perinuclear Golgi-like pattern and thought to be involved in hematopoietic and/or immune system processes.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9679 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse
Positive Control
WB: HEPA Whole Cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of SECTM1 using anti-SECTM1 antibody (PB9679).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: HEPA Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SECTM1 antigen affinity purified polyclonal antibody (Catalog # PB9679) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SECTM1 at approximately 23 kDa. The expected band size for SECTM1 is at 23 kDa.
Protein Target Info & Infographic
Gene/Protein Information For Sectm1b (Source: Uniprot.org, NCBI)
Gene Name
Sectm1b
Full Name
Secreted and transmembrane protein 1b
Weight
23477 MW
Superfamily
SECTM family
Alternative Names
Secreted and transmembrane protein 1b;Protein K-12;Sectm1b;K12, Sectm1;
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Sectm1b, check out the Sectm1b Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Sectm1b: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SECTM1/Sectm1b Antibody Picoband® (PB9679)
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No publications found for PB9679
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-SECTM1/Sectm1b Antibody Picoband®
Question
Please let us know what method you used to refine it.( chromatography etc…)
Verified customer
Asked: 2022-12-09
Answer
It is a rabbit anti-SECTM1 antigen affinity purified polyclonal antibody.
Boster Scientific Support
Answered: 2022-12-09
Question
I see that the anti-SECTM1/Sectm1b antibody PB9679 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-09-27
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for kidney using anti-SECTM1/Sectm1b antibody PB9679. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-07-31
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-31
Question
We are currently using anti-SECTM1/Sectm1b antibody PB9679 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says mouse. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-23
Answer
The anti-SECTM1/Sectm1b antibody (PB9679) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-23
Question
We are interested in to test anti-SECTM1/Sectm1b antibody PB9679 on mouse kidney for research purposes, then I may be interested in using anti-SECTM1/Sectm1b antibody PB9679 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
O. Li
Verified customer
Asked: 2015-03-26
Answer
The products we sell, including anti-SECTM1/Sectm1b antibody PB9679, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-03-26
