Anti-SEC61B Antibody Picoband™

Product Name

Anti-SEC61B Antibody Picoband™

View all SEC61B Antibodies

SKU/Catalog Number

A07334-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-SEC61B Antibody Picoband™ catalog # A07334-2. Tested in WB, FCM, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-SEC61B Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07334-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Immunogen

E.coli-derived human SEC61B recombinant protein (Position: R16-S96). Human SEC61B shares 98.8% amino acid (aa) sequence identity with mouse SEC61B.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A07334-2 is reactive to SEC61B in Human, Mouse, Rat

Applications

A07334-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

15 kDa

Calculated molecular weight

10 kDa

Background of SEC61B

Protein transport protein Sec61 subunit beta is a protein that in humans is encoded by the SEC61B gene. The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. Oligomers of the Sec61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane. This complex consists of three membrane proteins- alpha, beta, and gamma. This gene encodes the beta-subunit protein. The Sec61 subunits are also observed in the post-ER compartment, suggesting that these proteins can escape the ER and recycle back. There is evidence for multiple polyadenylated sites for this transcript.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg /1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of SEC61B using anti-SEC61B antibody (A07334-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human Hela whole cell lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: mouse C2C12 whole cell lysates,
Lane 7: mouse RAW264.7 whole cell lysates,
Lane 8: mouse 3T3-L1 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEC61B antigen affinity purified polyclonal antibody (Catalog # A07334-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEC61B at approximately 15 kDa. The expected band size for SEC61B is at 10 kDa.

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Figure 2. Flow Cytometry analysis of PC-3 cells using anti-SEC61B antibody (A07334-2).
Overlay histogram showing PC-3 cells stained with A07334-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SEC61B Antibody (A07334-2, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Gene/Protein Information For SEC61B (Source: Uniprot.org, NCBI)

Alternative Names

protein translocation complex beta; protein transport protein SEC61 beta subunit; protein transport protein Sec61 subunit beta; Sec61 beta subunit; Sec61 complex, beta subunit; SEC61B SEC61B SEC61 translocon subunit beta protein transport protein Sec61 subunit beta|SEC61 translocon beta subunit|Sec61 beta subunit|Sec61 complex, beta subunit|protein translocation complex beta|protein transport protein SEC61 beta subunit

For more info on SEC61B, check out the SEC61B Infographic

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In this infographic, you will see the following information for SEC61B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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