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Product Info Summary
| SKU: | A05847-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-SEC23B Antibody Picoband®
SKU/Catalog Number
A05847-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SEC23B Antibody Picoband® catalog # A05847-3. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SEC23B Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05847-3)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SEC23B recombinant protein (Position: R190-Q581).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A05847-3 is reactive to SEC23B in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
86 kDa
Calculated molecular weight
86.479kDa
Background of SEC23B
Protein transport protein Sec23B is a protein that in humans is encoded by the SEC23B gene. The protein encoded by this gene is a member of the SEC23 subfamily of the SEC23/SEC24 family, which is involved in vesicle trafficking. The encoded protein has similarity to yeast Sec23p component of COPII. COPII is the coat protein complex responsible for vesicle budding from the ER. The function of this gene product has been implicated in cargo selection and concentration. Multiple alternatively spliced transcript variants have been identified in this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05847-3 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human Raji whole cell, human HepG2 whole cell, human PC-3 whole cell, human A549 whole cell, human THP-1 whole cell, human HL-60 whole cell, rat liver tissue, rat stomach tissue, mouse liver tissue, mouse RAW2647 whole cell
FCM: SiHa cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SEC23B using anti-SEC23B antibody (A05847-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: human THP-1 whole cell lysates,
Lane 6: human HL-60 whole cell lysates,
Lane 7: rat liver tissue lysates,
Lane 8: rat stomach tissue lysates,
Lane 9: mouse liver tissue lysates,
Lane 10: mouse RAW264.7 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEC23B antigen affinity purified polyclonal antibody (Catalog # A05847-3) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SEC23B at approximately 86KD. The expected band size for SEC23B is at 86KD.
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Figure 2. Flow Cytometry analysis of SiHa cells using anti-SEC23B antibody (A05847-3).
Overlay histogram showing SiHa cells stained with A05847-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SEC23B Antibody (A05847-3, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For SEC23B (Source: Uniprot.org, NCBI)
Gene Name
SEC23B
Full Name
Protein transport protein Sec23B
Weight
86.479kDa
Superfamily
SEC23/SEC24 family
Alternative Names
T-lymphocyte surface antigen Ly-9; Cell surface molecule Ly-9; Lymphocyte antigen 9; SLAM family member 3; SLAMF3; Signaling lymphocytic activation molecule 3; CD229; LY9; CDABP0070 SEC23B CDA-II, CDAII, CDAN2, CWS7, HEMPAS, hSec23B SEC23 homolog B, COPII coat complex component protein transport protein Sec23B|SEC23 homolog B, coat complex II component|SEC23-like protein B|SEC23-related protein B|transport protein SEC23B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SEC23B, check out the SEC23B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SEC23B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SEC23B Antibody Picoband® (A05847-3)
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