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Product Info Summary
| SKU: | A30462 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB, IF (paraffin) |
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Product info
Product Name
Anti-SDCG1 Antibody
SKU/Catalog Number
A30462
Size
100ul
Form
Liquid
Description
Boster Bio Anti-SDCG1 Antibody (Catalog# A30462). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SDCG1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30462)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human SDCG1. Immunogen sequence location: 881-930
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A30462 is reactive to NEMF in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
122954 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30462 is guaranteed for ELISA, IHC-P, WB, IF (paraffin) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:20000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western blot analysis of lysates from HeLa and Jurkat cells, using SDCG1 Antibody. The lane on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For NEMF (Source: Uniprot.org, NCBI)
Gene Name
NEMF
Full Name
Nuclear export mediator factor NEMF
Weight
122954 MW
Superfamily
NEMF family
Alternative Names
NEMF;SDCCAG1;Nuclear export mediator factor NEMF;Antigen NY-CO-1;Serologically defined colon cancer antigen 1 NEMF IDDSAPN, NY-CO-1, SDCCAG1 nuclear export mediator factor nuclear export mediator factor NEMF| NY-CO-1|caliban homolog|serologically defined colon cancer 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NEMF, check out the NEMF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NEMF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SDCG1 Antibody (A30462)
Hello CJ!
No publications found for A30462
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-SDCG1 Antibody
Question
Is this A30462 anti-SDCG1 antibody reactive to the isotypes of NEMF?
D. Baker
Verified customer
Asked: 2019-09-12
Answer
The immunogen of A30462 anti-SDCG1 antibody is A peptide derived from human SDCG1. Immunogen sequence location: 881-930. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-12
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for astrocyte using anti-SDCG1 antibody A30462. Let me know if you need anything else.
L. Evans
Verified customer
Asked: 2018-08-14
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-08-14
Question
Does anti-SDCG1 antibody A30462 work on pig WB with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2018-05-02
Answer
Our lab technicians have not validated anti-SDCG1 antibody A30462 on pig. You can run a BLAST between pig and the immunogen sequence of anti-SDCG1 antibody A30462 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig leukemic t-cell in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-05-02
Question
We are currently using anti-SDCG1 antibody A30462 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?
A. Parker
Verified customer
Asked: 2015-10-08
Answer
The anti-SDCG1 antibody (A30462) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-10-08
Question
Is a blocking peptide available for product anti-SDCG1 antibody (A30462)?
W. Krishna
Verified customer
Asked: 2014-12-31
Answer
We do provide the blocking peptide for product anti-SDCG1 antibody (A30462). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-12-31
