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Product Info Summary
| SKU: | A01735-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Scn2a Antibody Picoband®
SKU/Catalog Number
A01735-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Scn2a Antibody Picoband® catalog # A01735-3. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Scn2a Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01735-3)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.005mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of mouse Scn2a, which shares 82.4% and 94.1% amino acid (aa) sequence identity with human and rat Scn2a, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01735-3 is reactive to Scn2a in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Observed Molecular Weight
320 kDa
Calculated molecular weight
57954 MW
Background of SCN2A
Navα1.2, also known as the sodium channel, voltage-gated, type II, alpha subunit is a protein that in humans is encoded by the SCN2A gene. Voltage-gated sodium channels are transmembrane glycoprotein complexes composed of a large alpha subunit with four repeat domains, each of which is composed of six membrane-spanning segments, and one or more regulatory beta subunits. Voltage-gated sodium channels function in the generation and propagation of action potentials in neurons and muscle. This gene encodes one member of the sodium channel alpha subunit gene family. Allelic variants of this gene are associated with seizure disorders and autism spectrum disorder. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01735-3 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Mouse, Rat
Positive Control
WB: rat brain tissue, mouse brain tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Scn2a using anti-Scn2a antibody (A01735-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Scn2a antigen affinity purified polyclonal antibody (Catalog # A01735-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Scn2a at approximately 320KD. The expected band size for Scn2a is at 320KD.
Protein Target Info & Infographic
Gene/Protein Information For Scn2a (Source: Uniprot.org, NCBI)
Gene Name
Scn2a
Full Name
Sodium channel protein type 2 subunit alpha
Weight
57954 MW
Superfamily
sodium channel (TC 1.A.1.10) family
Alternative Names
Fatty acid-binding protein, heart; Fatty acid-binding protein 3; Heart-type fatty acid-binding protein; H-FABP; Mammary-derived growth inhibitor; MDGI; Muscle fatty acid-binding protein; M-FABP; FABP3; FABP11; MDGI SCN2A BFIC3, BFIS3, BFNIS, DEE11, EA9, EIEE11, HBA, HBSCI, HBSCII, NAC2, Na(v)1.2, Nav1.21, SCN2A2, SCN2A sodium voltage-gated channel alpha subunit 2 sodium channel protein type 2 subunit alpha|sodium channel protein brain II subunit alpha|sodium channel protein type II subunit alpha|sodium channel protein, brain type 2 alpha subunit|sodium channel, voltage-gated, type II, alpha 1 polypeptide|sodium channel, voltage-gated, type II, alpha 2 polypeptide|sodium channel, voltage-gated, type II, alpha subunit|voltage-gated sodium channel subtype II|voltage-gated sodium channel subunit alpha Nav1.2|voltage-gated sodium channel type II alpha subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Scn2a, check out the Scn2a Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Scn2a: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Scn2a Antibody Picoband® (A01735-3)
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