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Product Info Summary
| SKU: | M10629 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-SCGN Monoclonal Antibody
View all Secretagogin Antibodies
SKU/Catalog Number
M10629
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-SCGN Monoclonal Antibody catalog # M10629. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SCGN Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M10629)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AFGD-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human SCGN Expressed at high levels in the pancreatic islets of Langerhans and to a much lesser extent in the gastrointestinal tract (stomach, small intestine and colon), the adrenal medulla and cortex and the thyroid C-cells.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M10629 is reactive to SCGN in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
140 kDa
Calculated molecular weight
70581 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M10629 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
IP 1:50
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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Western blot analysis of SCGN expression in human fetal brain lysate.
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
Click image to see more details
All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For SCGN (Source: Uniprot.org, NCBI)
Gene Name
SCGN
Full Name
Secretagogin
Weight
70581 MW
Alternative Names
5-aminolevulinate synthase, nonspecific, mitochondrial;ALAS-H;2.3.1.37;5-aminolevulinic acid synthase 1;Delta-ALA synthase 1;Delta-aminolevulinate synthase 1;ALAS1;ALAS3, ALASH;OK/SW-cl.121; SCGN CALBL, DJ501N12.8, SECRET, SEGN, setagin secretagogin, EF-hand calcium binding protein secretagogin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SCGN, check out the SCGN Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SCGN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SCGN Monoclonal Antibody (M10629)
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Customer Q&As
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4 Customer Q&As for Anti-SCGN Monoclonal Antibody
Question
See below the WB image, lot number and protocol we used for islet of langerhans using anti-SCGN Monoclonal antibody M10629. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-17
Question
My question regards to test anti-SCGN Monoclonal antibody M10629 on mouse islet of langerhans for research purposes, then I may be interested in using anti-SCGN Monoclonal antibody M10629 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-07
Answer
The products we sell, including anti-SCGN Monoclonal antibody M10629, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-07
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for islet of langerhans using anti-SCGN Monoclonal antibody M10629. Let me know if you need anything else.
A. Roberts
Verified customer
Asked: 2019-08-05
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-05
Question
I was wanting to use your anti-SCGN Monoclonal antibody for WB for mouse islet of langerhans on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse islet of langerhans identification?
Z. Brown
Verified customer
Asked: 2014-06-12
Answer
It shows on the product datasheet, M10629 anti-SCGN Monoclonal antibody has been tested for IP, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse islet of langerhans in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-06-12
