Product Info Summary
SKU: | A10769 |
---|---|
Size: | 100μl |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-SCAF1 Antibody
SKU/Catalog Number
A10769
Size
100μl
Form
Liquid
Description
Boster Bio Anti-SCAF1 Antibody catalog # A10769. Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SCAF1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10769)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human SFRS19. AA range:41-90
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A10769 is reactive to SCAF1 in Human, Mouse
Reconstitution
Calculated molecular weight
139270 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10769 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
Click image to see more details
Western Blot (WB) analysis of specific cells using SCAF1 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For SCAF1 (Source: Uniprot.org, NCBI)
Gene Name
SCAF1
Full Name
Splicing factor, arginine/serine-rich 19
Weight
139270 MW
Superfamily
splicing factor SR family
Alternative Names
Splicing factor, arginine/serine-rich 19;SR-related and CTD-associated factor 1;SR-related-CTD-associated factor;SCAF;Serine arginine-rich pre-mRNA splicing factor SR-A1;SR-A1;SCAF1;SFRS19, SRA1; Scaf1|Sr-a1|SR-related CTD-associated factor 1|splicing factor, arginine/serine-rich 19|CTD-binding SR-like protein rA1|CTD-binding SR-like rA1|SR-related and CTD-associated factor 1|serine arginine-rich pre-mRNA splicing factor SR-A1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SCAF1, check out the SCAF1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SCAF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SCAF1 Antibody (A10769)
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Customer Q&As
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6 Customer Q&As for Anti-SCAF1 Antibody
Question
We are currently using anti-SCAF1 antibody A10769 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-24
Answer
The anti-SCAF1 antibody (A10769) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-24
Question
See attached the WB image, lot number and protocol we used for heart left ventricle using anti-SCAF1 antibody A10769. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-07
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-07
Question
Is a blocking peptide available for product anti-SCAF1 antibody (A10769)?
Verified Customer
Verified customer
Asked: 2019-04-23
Answer
We do provide the blocking peptide for product anti-SCAF1 antibody (A10769). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-04-23
Question
I was wanting to use your anti-SCAF1 antibody for WB for mouse heart left ventricle on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse heart left ventricle identification?
Verified Customer
Verified customer
Asked: 2019-02-07
Answer
You can see on the product datasheet, A10769 anti-SCAF1 antibody has been tested for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse heart left ventricle in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-02-07
Question
Does anti-SCAF1 antibody A10769 work on goat WB with leukemic t-cell?
B. Williams
Verified customer
Asked: 2017-08-08
Answer
Our lab technicians have not validated anti-SCAF1 antibody A10769 on goat. You can run a BLAST between goat and the immunogen sequence of anti-SCAF1 antibody A10769 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat leukemic t-cell in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-08-08
Question
Would A10769 anti-SCAF1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
G. Patel
Verified customer
Asked: 2013-06-06
Answer
It shows on the product datasheet, A10769 anti-SCAF1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-06-06