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Product Info Summary
| SKU: | A09641-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-SARDH Antibody Picoband®
SKU/Catalog Number
A09641-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SARDH Antibody Picoband® catalog # A09641-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-SARDH Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09641-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SARDH recombinant protein (Position: M1-E640).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A09641-1 is reactive to SARDH in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
101 kDa
Calculated molecular weight
101.037kDa
Background of SARDH
Sarcosine dehydrogenase, mitochondrial is an enzyme that in humans is encoded by the SARDH gene. This gene encodes an enzyme localized to the mitochondrial matrix which catalyzes the oxidative demethylation of sarcosine. This enzyme is distinct from another mitochondrial matrix enzyme, dimethylglycine dehydrogenase, which catalyzes a reaction resulting in the formation of sarcosine. Mutations in this gene are associated with sarcosinemia. Alternatively spliced transcript variants have been described.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09641-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human hepatocellular carcinoma tumor tissue (HCCT), human hepatocellular carcinoma paracancerous tissue (HCCP), ran pancreas tissue, rat liver tissue, rat kidney tissue, rat RH35 whole cell, mouse pancreas tissue, mouse liver tissue, mouse kidney tissue
FCM: U87 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SARDH using anti-SARDH antibody (A09641-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates,
Lane 2: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates,
Lane 3: ran pancreas tissue lysates,
Lane 4: rat liver tissue lysates,
Lane 5: rat kidney tissue lysates,
Lane 6: rat RH35 whole cell lysates,
Lane 7: mouse pancreas tissue lysates,
Lane 8: mouse liver tissue lysates,
Lane 9: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SARDH antigen affinity purified polyclonal antibody (Catalog # A09641-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SARDH at approximately 101 kDa. The expected band size for SARDH is at 101 kDa.
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Figure 2. Flow Cytometry analysis of U87 cells using anti-SARDH antibody (A09641-1).
Overlay histogram showing U87 cells stained with A09641-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SARDH Antibody (A09641-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For SARDH (Source: Uniprot.org, NCBI)
Gene Name
SARDH
Full Name
Sarcosine dehydrogenase, mitochondrial
Weight
101.037kDa
Superfamily
GcvT family
Alternative Names
Band 4.1-like protein 5; EPB41L5; KIAA1548 SARDH BPR-2, DMGDHL1, SAR, SARD, SDH sarcosine dehydrogenase sarcosine dehydrogenase, mitochondrial|dimethylglycine dehydrogenase-like 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SARDH, check out the SARDH Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SARDH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SARDH Antibody Picoband® (A09641-1)
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