Product Info Summary
SKU: | A02503 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, IHC, WB |
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Product info
Product Name
Anti-S100 A1 Antibody
SKU/Catalog Number
A02503
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-S100 A1 Antibody (Catalog # A02503). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-S100 A1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02503)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human S100 A1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02503 is reactive to S100A1 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
10546 MW
Background of S100a1
Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity.
Patrick Most, J. Biol. Chem., Nov 2003; 278: 48404 - 48412.
Miki Okada, J. Biol. Chem., Feb 2004; 279: 4221 - 4233.
Melanie Boerries, Mol. Cell. Biol., Jun 2007; 27: 4365 - 4373.
Patrick Most, J. Clin. Invest., Dec 2004; 114: 1550 - 1563.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02503 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500
Validation Images & Assay Conditions
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Western blot analysis of extracts from 293 cells (Lane 2), using S100 A1 antiobdy A02503. The lane on the left is treated with synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded human brain tissue using S100 A1 antibody A02503.
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Western blot analysis of extracts from A549 cells, using S100 A1 antibody A02503.
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Immunofluorescence analysis of A549 cells, using S100 A1 antibody A02503.
Protein Target Info & Infographic
Gene/Protein Information For S100A1 (Source: Uniprot.org, NCBI)
Gene Name
S100A1
Full Name
Protein S100-A1
Weight
10546 MW
Superfamily
S-100 family
Alternative Names
Protein S100-A1;S-100 protein alpha chain;S-100 protein subunit alpha;S100 calcium-binding protein A1;S100A1;S100A; S100A1 S100, S100-alpha, S100A S100 calcium binding protein A1 protein S100-A1|S-100 protein alpha chain|S-100 protein subunit alpha|S100 alpha|S100 protein, alpha polypeptide
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on S100A1, check out the S100A1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for S100A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-S100 A1 Antibody (A02503)
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