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Product Info Summary
| SKU: | A08744 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-RWDD3 Antibody
SKU/Catalog Number
A08744
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-RWDD3 Antibody (Catalog # A08744). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
RWDD3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-RWDD3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08744)
Host
Rabbit
Contents
RWDD3 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
RWDD3 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human RWDD3. The immunogen is located within the last 50 amino acids of RWDD3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Three isoforms of LRRFIP2 are known to exist; this antibody will recognize all three. LRRFIP2 antibody is predicted to not cross-react with other LRRFIP family members.
Reactive Species
A08744 is reactive to RWDD3 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
30543 MW
Background of RWDD3
RWDD3 (RSUME), a small RWD-containing protein, has a central role in sumoylation by enhancing SUMO conjugation in the regulatory network of immune-inflammatory signals. RWDD3 increases IκBeta sumoylation and stability. In addition, RWDD3 inhibits TNF-α-induced kappaB-LUC (Luciferase) reporter activity, showing the functional consequence of IκBeta increased stability. RSUME-enhanced sumoylation of IκBeta leads to the inhibition of NF-κBeta activity on two well-known inflammatory genes, IL-8 and cyclooxygenase-2 (Cox-2) and therefore may also favor anti-inflammatory pathways. Expression of RWDD3 was induced under hypoxic conditions and it has a potential role during vascularization. Both BMP-4 and RWDD3 may be interesting targets for inhibiting steps involved in pituitary tumorigenesis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08744 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
RWDD3 antibody can be used for detection of RWDD3 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of RWDD3 in rat kidney tissue lysate with RWDD3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Protein Target Info & Infographic
Gene/Protein Information For RWDD3 (Source: Uniprot.org, NCBI)
Gene Name
RWDD3
Full Name
RWD domain-containing protein 3
Weight
30543 MW
Alternative Names
RSUME, RSUME, RWD domain-containing protein 3, RWD-containing sumoylation enhancer RWDD3 RSUME RWD domain containing 3 RWD domain-containing protein 3|RWD domain-containing sumoylation enhancer|RWD-containing sumoylation enhancer
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RWDD3, check out the RWDD3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RWDD3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RWDD3 Antibody (A08744)
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