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Product Info Summary
| SKU: | A31740 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-RTL10 Antibody Picoband®
SKU/Catalog Number
A31740
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-RTL10 Antibody Picoband® catalog # A31740. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RTL10 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A31740)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human RTL10.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A31740 is reactive to RTL10 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
36-39 kDa
Calculated molecular weight
14377 MW
Background of RTL10
RTL10 (Retrotransposon Gag Like 10) is a Protein Coding gene. This gene is mapped to 22q11.21. It could induce apoptosis in a BH3 domain-dependent manner. The direct interaction network of Bcl-2 family members may play a key role in modulation RTL10/BOP intrinsic apoptotic signaling activity. Diseases associated with RTL10 include Hemophagocytic Lymphohistiocytosis, Familial, 3.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A31740 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
"Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
"
Positive Control
WB: human placenta tissue, human A431 whole cell, human HL-60 whole cell, human U2OS whole cell, human PC-3 whole cell, rat lung tissue, mouse HEPA1-6 whole cell
FCM: HL-60 cell, PC-3 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of RTL10 using anti-RTL10 antibody (A31740).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human HL-60 whole cell lysates,
Lane 4: human U2OS whole cell lysates,
Lane 5: human PC-3 whole cell lysates,
Lane 6: rat lung tissue lysates,
Lane 7: mouse HEPA1-6 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RTL10 antigen affinity purified polyclonal antibody (Catalog # A31740) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. Specific bands were detected for RTL10 at approximately 36-39KD. The expected band size for RTL10 is at 39KD.
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Figure 2. Flow Cytometry analysis of HL-60 cells using anti-RTL10 antibody (A31740).
Overlay histogram showing HL-60 cells stained with A31740 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RTL10 Antibody (A31740, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Figure 3. Flow Cytometry analysis of PC-3 cells using anti-RTL10 antibody (A31740).
Overlay histogram showing PC-3 cells stained with A31740 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RTL10 Antibody (A31740, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For RTL10 (Source: Uniprot.org, NCBI)
Gene Name
RTL10
Full Name
Protein Bop
Weight
14377 MW
Alternative Names
Protein Bop; BH3-only protein; Retrotransposon Gag-like protein 10; RTL10; BOP, C22orf29 RTL10 BOP, C22orf29 retrotransposon Gag like 10 protein Bop|BH3-only protein|retrotransposon Gag-like protein 10
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RTL10, check out the RTL10 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RTL10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RTL10 Antibody Picoband® (A31740)
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Customer Q&As
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1 Customer Q&As for Anti-RTL10 Antibody Picoband®
Question
We are currently using anti-RTL10 antibody A31740 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on horse tissues as well?
B. Jackson
Verified customer
Asked: 2020-03-17
Answer
The anti-RTL10 antibody (A31740) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-17
