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Product Info Summary
| SKU: | A07282-2 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Rsl1d1 Antibody Picoband®
SKU/Catalog Number
A07282-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-Rsl1d1 Antibody Picoband® catalog # A07282-2. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Rsl1d1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07282-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Rsl1d1 recombinant protein (Position: E62-N452).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A07282-2 is reactive to Rsl1d1 in Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
65 kDa
Calculated molecular weight
67166 MW
Background of RSL1D1
Ribosomal L1 domain-containing protein 1 is a protein that in humans is encoded by the RSL1D1 gene. Predicted to enable mRNA 3'-UTR binding activity and mRNA 5'-UTR binding activity. Predicted to be involved in maturation of LSU-rRNA; regulation of apoptotic process; and regulation of cellular senescence. Predicted to act upstream of or within regulation of protein localization. Located in nucleolus. Is expressed in several structures, including eye; genitourinary system; liver; lung; and spleen. Orthologous to human RSL1D1 (ribosomal L1 domain containing 1).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07282-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25 µg/ml, Mouse, Rat
ELISA, 0.1-0.5 µg/ml, Mouse
Positive Control
WB: rat brain tissue, rat kidney tissue, rat heart tissue, rat RH35 whole cell, mouse brain tissue, mouse heart tissue, mouse C2C12 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Rsl1d1 using anti-Rsl1d1 antibody (A07282-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat kidney tissue lysates,
Lane 3: rat heart tissue lysates,
Lane 4: rat RH35 whole cell lysates,
Lane 5: mouse brain tissue lysates,
Lane 6: mouse heart tissue lysates,
Lane 7: mouse C2C12 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Rsl1d1 antigen affinity purified polyclonal antibody (Catalog # A07282-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Rsl1d1 at approximately 65 kDa. The expected band size for Rsl1d1 is at 54,55 kDa.
Protein Target Info & Infographic
Gene/Protein Information For Rsl1d1 (Source: Uniprot.org, NCBI)
Gene Name
Rsl1d1
Full Name
Ribosomal L1 domain-containing protein 1
Weight
67166 MW
Superfamily
universal ribosomal protein uL1 family
Alternative Names
Interleukin-18-binding protein; IL-18BP; Interferon gamma-inducing factor-binding protein; Il18bp; Igifbp RSL1D1 CSIG, L12, PBK1, UTP30 ribosomal L1 domain containing 1 ribosomal L1 domain-containing protein 1|CATX-11|cellular senescence-inhibited gene protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Rsl1d1, check out the Rsl1d1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Rsl1d1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Rsl1d1 Antibody Picoband® (A07282-2)
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