Product Info Summary
SKU: | A05831-1 |
---|---|
Size: | 100 µg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-RPS17 Antibody Picoband™
SKU/Catalog Number
A05831-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-RPS17 Antibody Picoband™ catalog # A05831-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-RPS17 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05831-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human RPS17 recombinant protein (Position: M1-V135).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A05831-1 is reactive to RPS17 in Human, Mouse, Rat
Applications
A05831-1 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
18 kDa
Calculated molecular weight
Background of RPS17
40S ribosomal protein S17 is a protein that in humans is encoded by the RPS17 gene. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of four RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S17E family of ribosomal proteins and is located in the cytoplasm. Mutations in this gene cause Diamond-Blackfan anemia 4. Alternative splicing of this gene results in multiple transcript variants. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
![a05831 1 rps17 primary antibodies wb testing 1 a05831 1 rps17 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a05831-1-rps17-primary-antibodies-wb-testing-1.jpg)
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Figure 1. Western blot analysis of RPS17 using anti-RPS17 antibody (A05831-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human THP-1 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human placenta tissue lysates,
Lane 5: rat ovary tissue lysates,
Lane 6: rat RH35 whole cell lysates,
Lane 7: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS17 antigen affinity purified polyclonal antibody (Catalog # A05831-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPS17 at approximately 18 kDa. The expected band size for RPS17 is at 16 kDa.
![a05831 1 rps17 primary antibodies if testing 2 a05831 1 rps17 primary antibodies if testing 2](https://www.bosterbio.com/media/catalog/product/a/0/a05831-1-rps17-primary-antibodies-if-testing-2.jpg)
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Figure 2. IF analysis of RPS17 using anti-RPS17 antibody (A05831-1).
RPS17 was detected in an immunocytochemical section of PC3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RPS17 Antibody (A05831-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
![a05831 1 rps17 primary antibodies fcm testing 3 a05831 1 rps17 primary antibodies fcm testing 3](https://www.bosterbio.com/media/catalog/product/a/0/a05831-1-rps17-primary-antibodies-fcm-testing-3.png)
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Figure 3. Flow Cytometry analysis of A431 cells using anti-RPS17 antibody (A05831-1).
Overlay histogram showing A431 cells stained with A05831-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPS17 Antibody (A05831-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For RPS17 (Source: Uniprot.org, NCBI)
Gene Name
RPS17
Full Name
40S ribosomal protein S17
Weight
Superfamily
eukaryotic ribosomal protein eS17 family
Alternative Names
40S ribosomal protein S17; DBA4; ribosomal protein S17; RPS17L1RPS17L2MGC72007 RPS17 DBA4L, RPS17L1, RPS17L2, S17, RPS17 ribosomal protein S17 40S ribosomal protein S17|small ribosomal subunit protein eS17
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RPS17, check out the RPS17 Infographic
![RPS17 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RPS17: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RPS17 Antibody Picoband™ (A05831-1)
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