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Product Info Summary
| SKU: | M05175 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, WB |
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Product info
Product Name
Anti-RPL4 Antibody (N-Term)
SKU/Catalog Number
M05175
Size
50 µl
Description
Boster Bio Anti-RPL4 Antibody (N-Term) (Catalog # M05175). Tested in WB, Flow Cytometry, IF application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-RPL4 Antibody (N-Term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05175)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This RPL4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 119-149 amino acids from human RPL4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M05175 is reactive to RPL4 in Human
Reconstitution
Calculated molecular weight
47697 Da
Background of RPL4
Protein transport. Probably involved in vesicular traffic (By similarity).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05175 is guaranteed for Flow Cytometry, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:2000
FC: 1:25
Validation Images & Assay Conditions
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human osteosarcoma cell line) cells labeling RPL4 with M05175 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and nucleus staining on U-2 OS cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
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All lanes : Anti-RPL4 Antibody (N-Term) at 1:2000 dilution
Lane 1: Hela whole cell lysate
Lane 2: K562 whole cell lysate
Lane 3: Jurkat whole cell lysate
Lane 4: Raji whole cell lysate
Lane 5: NCI-H929 whole cell lysate
Lane 6: MCF-7 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 48 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Overlay histogram showing U-2OS cells stained with M05175 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (M05175, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Protein Target Info & Infographic
Gene/Protein Information For RPL4 (Source: Uniprot.org, NCBI)
Gene Name
RPL4
Full Name
60S ribosomal protein L4
Weight
47697 Da
Superfamily
universal ribosomal protein uL4 family
Alternative Names
60S ribosomal protein L4, 60S ribosomal protein L1, RPL4, RPL1 RPL4 L4 ribosomal protein L4 60S ribosomal protein L4|60S ribosomal protein L1|large ribosomal subunit protein uL4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RPL4, check out the RPL4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RPL4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RPL4 Antibody (N-Term) (M05175)
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No publications found for M05175
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