Anti-RPL35/Ribosomal Protein L35 Antibody

RPL35 antibody

Boster Bio Anti-RPL35/Ribosomal Protein L35 Antibody (Catalog # A10561). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A10561
Size: 100 µl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

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Product Name

Anti-RPL35/Ribosomal Protein L35 Antibody

View all RPL35 Antibodies

SKU/Catalog Number

A10561

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-RPL35/Ribosomal Protein L35 Antibody (Catalog # A10561). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-RPL35/Ribosomal Protein L35 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10561)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human RPL35.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A10561 is reactive to RPL35 in Human, Mouse, Rat

Applications

A10561 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

14551 MW

Background of RPL35

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L29P family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Patel S.K., Submitted (JUL-1994) to the EMBL/GenBank/DDBJ databases
Ebert L., Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Humphray S.J., Nature 429:369-374(2004).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

Gene/Protein Information For RPL35 (Source: Uniprot.org, NCBI)

Gene Name

RPL35

Full Name

60S ribosomal protein L35

Weight

14551 MW

Superfamily

universal ribosomal protein uL29 family

Alternative Names

M; ribosomal protein L35,60S ribosomal protein L35 RPL35 DBA19, L35 ribosomal protein L35 60S ribosomal protein L35|large ribosomal subunit protein uL29

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on RPL35, check out the RPL35 Infographic

RPL35 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for RPL35: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-RPL35/Ribosomal Protein L35 Antibody

Question

I would like to test anti-RPL35/Ribosomal Protein L35 antibody A10561 on human brain for research purposes, then I may be interested in using anti-RPL35/Ribosomal Protein L35 antibody A10561 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-05-07

Answer

The products we sell, including anti-RPL35/Ribosomal Protein L35 antibody A10561, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-05-07

Question

Does anti-RPL35/Ribosomal Protein L35 antibody A10561 work on feline WB with lung?

Verified Customer

Verified customer

Asked: 2020-04-22

Answer

Our lab technicians have not validated anti-RPL35/Ribosomal Protein L35 antibody A10561 on feline. You can run a BLAST between feline and the immunogen sequence of anti-RPL35/Ribosomal Protein L35 antibody A10561 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline lung in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-04-22

Question

We are currently using anti-RPL35/Ribosomal Protein L35 antibody A10561 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2019-11-28

Answer

The anti-RPL35/Ribosomal Protein L35 antibody (A10561) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-11-28

Question

Is this A10561 anti-RPL35/Ribosomal Protein L35 antibody reactive to the isotypes of RPL35?

K. Carter

Verified customer

Asked: 2019-01-15

Answer

The immunogen of A10561 anti-RPL35/Ribosomal Protein L35 antibody is Synthesized peptide derived from internal of human RPL35. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-01-15

Order DetailsPrice
A10561-100uL

100uL

$360

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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