Product Info Summary
SKU: | A10561 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-RPL35/Ribosomal Protein L35 Antibody
SKU/Catalog Number
A10561
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-RPL35/Ribosomal Protein L35 Antibody (Catalog # A10561). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RPL35/Ribosomal Protein L35 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10561)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human RPL35.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A10561 is reactive to RPL35 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
14551 MW
Background of RPL35
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L29P family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Patel S.K., Submitted (JUL-1994) to the EMBL/GenBank/DDBJ databases
Ebert L., Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Humphray S.J., Nature 429:369-374(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10561 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from HeLa cells, Jurkat cells, COLO cells and 293 cells, using RPL35 antibody A10561.
Protein Target Info & Infographic
Gene/Protein Information For RPL35 (Source: Uniprot.org, NCBI)
Gene Name
RPL35
Full Name
60S ribosomal protein L35
Weight
14551 MW
Superfamily
universal ribosomal protein uL29 family
Alternative Names
60S ribosomal protein L35;RPL35; RPL35 DBA19, L35 ribosomal protein L35 60S ribosomal protein L35|large ribosomal subunit protein uL29
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RPL35, check out the RPL35 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RPL35: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RPL35/Ribosomal Protein L35 Antibody (A10561)
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No publications found for A10561
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Customer Q&As
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4 Customer Q&As for Anti-RPL35/Ribosomal Protein L35 Antibody
Question
I would like to test anti-RPL35/Ribosomal Protein L35 antibody A10561 on human brain for research purposes, then I may be interested in using anti-RPL35/Ribosomal Protein L35 antibody A10561 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-05-07
Answer
The products we sell, including anti-RPL35/Ribosomal Protein L35 antibody A10561, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-05-07
Question
Does anti-RPL35/Ribosomal Protein L35 antibody A10561 work on feline WB with lung?
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
Our lab technicians have not validated anti-RPL35/Ribosomal Protein L35 antibody A10561 on feline. You can run a BLAST between feline and the immunogen sequence of anti-RPL35/Ribosomal Protein L35 antibody A10561 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline lung in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-22
Question
We are currently using anti-RPL35/Ribosomal Protein L35 antibody A10561 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-28
Answer
The anti-RPL35/Ribosomal Protein L35 antibody (A10561) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-28
Question
Is this A10561 anti-RPL35/Ribosomal Protein L35 antibody reactive to the isotypes of RPL35?
K. Carter
Verified customer
Asked: 2019-01-15
Answer
The immunogen of A10561 anti-RPL35/Ribosomal Protein L35 antibody is Synthesized peptide derived from internal of human RPL35. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-01-15