Product Info Summary
SKU: | A07057-3 |
---|---|
Size: | 100 µg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-RPL18 Antibody Picoband™
SKU/Catalog Number
A07057-3
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-RPL18 Antibody Picoband™ catalog # A07057-3. Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-RPL18 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07057-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
IgG
Immunogen
E.coli-derived human RPL18 recombinant protein (Position: K19-A177).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A07057-3 is reactive to RPL18 in Human, Mouse, Rat
Applications
A07057-3 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
23 kDa
Calculated molecular weight
52588 MW
Background of RPL18
60S ribosomal protein L18 is a protein that in humans is encoded by the RPL18 gene. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a member of the L18E family of ribosomal proteins that is a component of the 60S subunit. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Immunohistochemistry, 2-5 µg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human
ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
![a07057 3 rpl18 primary antibodies wb testing 1 a07057 3 rpl18 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a07057-3-rpl18-primary-antibodies-wb-testing-1.jpg)
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Figure 1. Western blot analysis of RPL18 using anti-RPL18 antibody (A07057-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human Daudi whole cell lysates,
Lane 5: rat testis tissue lysates,
Lane 6: rat brain tissue lysates,
Lane 7: mouse testis tissue lysates,
Lane 8: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPL18 antigen affinity purified polyclonal antibody (Catalog # A07057-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPL18 at approximately 23 kDa. The expected band size for RPL18 is at 22 kDa.
![a07057 3 rpl18 primary antibodies ihc testing 2 a07057 3 rpl18 primary antibodies ihc testing 2](https://www.bosterbio.com/media/catalog/product/a/0/a07057-3-rpl18-primary-antibodies-ihc-testing-2.jpg)
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Figure 2. IHC analysis of RPL18 using anti-RPL18 antibody (A07057-3).
RPL18 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPL18 Antibody (A07057-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a07057 3 rpl18 primary antibodies ihc testing 3 a07057 3 rpl18 primary antibodies ihc testing 3](https://www.bosterbio.com/media/catalog/product/a/0/a07057-3-rpl18-primary-antibodies-ihc-testing-3.jpg)
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Figure 3. IHC analysis of RPL18 using anti-RPL18 antibody (A07057-3).
RPL18 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPL18 Antibody (A07057-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a07057 3 rpl18 primary antibodies ihc testing 4 a07057 3 rpl18 primary antibodies ihc testing 4](https://www.bosterbio.com/media/catalog/product/a/0/a07057-3-rpl18-primary-antibodies-ihc-testing-4.jpg)
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Figure 4. IHC analysis of RPL18 using anti-RPL18 antibody (A07057-3).
RPL18 was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPL18 Antibody (A07057-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a07057 3 rpl18 primary antibodies ihc testing 5 a07057 3 rpl18 primary antibodies ihc testing 5](https://www.bosterbio.com/media/catalog/product/a/0/a07057-3-rpl18-primary-antibodies-ihc-testing-5.jpg)
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Figure 5. IHC analysis of RPL18 using anti-RPL18 antibody (A07057-3).
RPL18 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPL18 Antibody (A07057-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a07057 3 rpl18 primary antibodies ihc testing 6 a07057 3 rpl18 primary antibodies ihc testing 6](https://www.bosterbio.com/media/catalog/product/a/0/a07057-3-rpl18-primary-antibodies-ihc-testing-6.jpg)
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Figure 6. IHC analysis of RPL18 using anti-RPL18 antibody (A07057-3).
RPL18 was detected in a paraffin-embedded section of human testicular germ cell tumors tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPL18 Antibody (A07057-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a07057 3 rpl18 primary antibodies ihc testing 7 a07057 3 rpl18 primary antibodies ihc testing 7](https://www.bosterbio.com/media/catalog/product/a/0/a07057-3-rpl18-primary-antibodies-ihc-testing-7.jpg)
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Figure 7. IHC analysis of RPL18 using anti-RPL18 antibody (A07057-3).
RPL18 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPL18 Antibody (A07057-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a07057 3 rpl18 primary antibodies ihc testing 8 a07057 3 rpl18 primary antibodies ihc testing 8](https://www.bosterbio.com/media/catalog/product/a/0/a07057-3-rpl18-primary-antibodies-ihc-testing-8.jpg)
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Figure 8. IHC analysis of RPL18 using anti-RPL18 antibody (A07057-3).
RPL18 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RPL18 Antibody (A07057-3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a07057 3 rpl18 primary antibodies if testing 9 a07057 3 rpl18 primary antibodies if testing 9](https://www.bosterbio.com/media/catalog/product/a/0/a07057-3-rpl18-primary-antibodies-if-testing-9.jpg)
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Figure 9. IF analysis of RPL18 using anti-RPL18 antibody (A07057-3).
RPL18 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RPL18 Antibody (A07057-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
![a07057 3 rpl18 primary antibodies fcm testing 10 a07057 3 rpl18 primary antibodies fcm testing 10](https://www.bosterbio.com/media/catalog/product/a/0/a07057-3-rpl18-primary-antibodies-fcm-testing-10.png)
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Figure 10. Flow Cytometry analysis of Hela cells using anti-RPL18 antibody (A07057-3).
Overlay histogram showing Hela cells stained with A07057-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPL18 Antibody (A07057-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For RPL18 (Source: Uniprot.org, NCBI)
Gene Name
RPL18
Full Name
60S ribosomal protein L18
Weight
52588 MW
Superfamily
eukaryotic ribosomal protein eL18 family
Alternative Names
ribosomal protein L18,60S ribosomal protein L18 RPL18 DBA18, L18 ribosomal protein L18 60S ribosomal protein L18|large ribosomal subunit protein eL18
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RPL18, check out the RPL18 Infographic
![RPL18 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RPL18: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RPL18 Antibody Picoband™ (A07057-3)
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