Anti-ROR1 Antibody

Product Name

Anti-ROR1 Antibody

View all ROR1 Antibodies

SKU/Catalog Number

A03192

Size

50 µl

Description

Boster Bio Anti-ROR1 Antibody (Catalog # A03192). Tested in IHC-P, WB, Flow Cytometry application(s). This antibody reacts with Human, Mouse.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-ROR1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03192)

Host

Rabbit

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

This ROR1 antibody is generated from rabbits immunized with recombinant human ROR1 protein (aa region: 112 - 399).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A03192 is reactive to ROR1 in Human, Mouse

Applications

A03192 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

104283 Da

Background of ROR1

ROR1 is a receptor protein tyrosine kinase whose cellular role has not been determined. It is a type I membrane protein and belongs to the ROR subfamily of cell surface receptors. Studies of a similar protein in mouse suggest that this protein may interact with another receptor protein tyrosine kinase and may be involved in skeletal and cardiac development.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:4000
IHC-P: 1:100
FC: 1:25

Validation Images & Assay Conditions

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All lanes : Anti-ROR1 Antibody at 1:4000 dilution
Lane 1: K562 whole cell lysate
Lane 2: MDA-MB-231 whole cell lysate
Lane 3: NCI-H226 whole cell lysate
Lane 4: A549 whole cell lysate
Lane 5: NIH/3T3 whole cell lysate
Lane 6: Mouse kidney tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 104 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

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Anti-ROR1 Antibody at 1:4000 dilution + PANC-1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 104 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

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Immunohistochemical analysis of A03192 on paraffin-embedded Human heart tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

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Immunohistochemical analysis of A03192 on paraffin-embedded Human kidney tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

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Overlay histogram showing A549 cells stained with A03192 (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (A03192, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Gene/Protein Information For ROR1 (Source: Uniprot.org, NCBI)

Alternative Names

EC 2.7.10.1; MGC99659; neurotrophic tyrosine kinase receptor-related 1; Neurotrophic tyrosine kinase, receptor-related 1; NTRKR1; NTRKR1dJ537F10.1; receptor tyrosine kinase-like orphan receptor 1; ROR1; tyrosine-protein kinase transmembrane receptor ROR1 ROR1 NTRKR1, dJ537F10.1 receptor tyrosine kinase like orphan receptor 1 inactive tyrosine-protein kinase transmembrane receptor ROR1|neurotrophic tyrosine kinase, receptor-related 1

For more info on ROR1, check out the ROR1 Infographic

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In this infographic, you will see the following information for ROR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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