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Product Info Summary
| SKU: | M01023 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-ROCK2 Monoclonal Antibody
SKU/Catalog Number
M01023
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-ROCK2 Monoclonal Antibody catalog # M01023. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ROCK2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01023)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ACDH-18
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human ROCK2 Regulates the assembly of the actin cytoskeleton. Promotes formation of stress fibers and of focal adhesion complexes. Plays a role in smooth muscle contraction.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01023 is reactive to ROCK2 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
161 kDa
Calculated molecular weight
22088 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01023 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
ICC/IF 1:50-1:200
Positive Control
WB: human Hela whole cell, human HepG2 whole cell, human MCF-7 whole cell, human SH-SY5Y whole cell, rat lung tissue, rat brain tissue, mouse lung tissue, mouse brain tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ROCK2 using anti-ROCK2 antibody (M01023).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human SH-SY5Y whole cell lysates,
Lane 5: rat lung tissue lysates,
Lane 6: rat brain tissue lysates,
Lane 7: mouse lung tissue lysates,
Lane 8: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROCK2 antigen affinity purified monoclonal antibody (Catalog # M01023) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ROCK2 at approximately 161 kDa. The expected band size for ROCK2 is at 161 kDa.
Protein Target Info & Infographic
Gene/Protein Information For ROCK2 (Source: Uniprot.org, NCBI)
Gene Name
ROCK2
Full Name
Rho-associated protein kinase 2
Weight
22088 MW
Superfamily
protein kinase superfamily
Alternative Names
Glutathione peroxidase 1;GPx-1;GSHPx-1;1.11.1.9;Cellular glutathione peroxidase;GPX1; ROCK2 ROCK-II Rho associated coiled-coil containing protein kinase 2 rho-associated protein kinase 2|p164 ROCK-2|rho-associated, coiled-coil-containing protein kinase II
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ROCK2, check out the ROCK2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ROCK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ROCK2 Monoclonal Antibody (M01023)
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