Anti-RNUXA PHAX Antibody

Product Name

Anti-RNUXA PHAX Antibody

View all PHAX Antibodies

SKU/Catalog Number

A08772

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-RNUXA PHAX Antibody (Catalog # A08772). Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-RNUXA PHAX Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08772)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human RNUXA.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A08772 is reactive to PHAX in Human

Reconstitution

Calculated molecular weight

44403 MW

Background of PHAX

A phosphoprotein adapter involved in the XPO1-mediated U snRNA export from the nucleus. Bridge components required for U snRNA export, the cap binding complex (CBC)-bound snRNA on the one hand and the GTPase Ran in its active GTP-bound form together with the export receptor XPO1 on the other. Its phosphorylation in the nucleus is required for U snRNA export complex assembly and export, while its dephosphorylation in the cytoplasm causes export complex disassembly. It is recycled back to the nucleus via the importin alpha/beta heterodimeric import receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Its compartmentalized phosphorylation cycle may also contribute to the directionality of export. Binds strongly to m7G-capped U1 and U5 small nuclear RNAs (snRNAs) in a sequence-unspecific manner and phosphorylation-independent manner By similarity. Plays also a role in the biogenesis of U3 small nucleolar RNA (snoRNA). Involved in the U3 snoRNA transport from nucleoplasm to Cajal bodies. Binds strongly to m7G-capped U3, U8 and U13 precursor snoRNAs and weakly to trimethylated (TMG)-capped U3, U8 and U13 snoRNAs. Binds also to telomerase RNA.

Ota T., Nat. Genet. 36:40-45(2004).
Ebert L., Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Boulon S., Mol. Cell 16:777-787(2004).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A08772 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

Gene/Protein Information For PHAX (Source: Uniprot.org, NCBI)

Alternative Names

Phosphorylated adapter RNA export protein;RNA U small nuclear RNA export adapter protein;PHAX;RNUXA; PHAX RNUXA phosphorylated adaptor for RNA export phosphorylated adapter RNA export protein|RNA U small nuclear RNA export adapter protein|RNA U, small nuclear RNA export adapter (phosphorylation regulated)|RNA U, small nuclear RNA export adaptor (phosphorylation regulated)

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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