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Product Info Summary
| SKU: | A07752-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-RNMT Antibody Picoband®
SKU/Catalog Number
A07752-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-RNMT Antibody Picoband® catalog # A07752-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-RNMT Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07752-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human RNMT recombinant protein (Position: R197-E380).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A07752-1 is reactive to RNMT in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
56 kDa
Calculated molecular weight
49229 MW
Background of RNMT
Enables RNA binding activity and mRNA (guanine-N7-)-methyltransferase activity. Involved in 7-methylguanosine mRNA capping. Located in fibrillar center and nucleoplasm. Part of mRNA cap binding activity complex; mRNA cap methyltransferase complex; and receptor complex.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07752-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Hela whole cell, human U2OS whole cell, human MOLT-4 whole cell
FCM: Daudi cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of RNMT using anti-RNMT antibody (A07752-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human U2OS whole cell lysates,
Lane 3: human MOLT-4 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNMT antigen affinity purified polyclonal antibody (Catalog # A07752-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNMT at approximately 56 kDa. The expected band size for RNMT is at 55 kDa.
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Figure 2. Flow Cytometry analysis of Daudi cells using anti-RNMT antibody (A07752-1).
Overlay histogram showing Daudi cells stained with A07752-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RNMT Antibody (A07752-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For RNMT (Source: Uniprot.org, NCBI)
Gene Name
RNMT
Full Name
mRNA cap guanine-N7 methyltransferase
Weight
49229 MW
Superfamily
class I-like SAM-binding methyltransferase superfamily
Alternative Names
Protein SOX-15; Protein SOX-12; Protein SOX-20; SOX15; SOX12; SOX20; SOX26; SOX27 RNMT CMT1, CMT1c, MET, Met, RG7MT1, cm1p, hCMT1, hMet RNA guanine-7 methyltransferase mRNA cap guanine-N7 methyltransferase|RNA (guanine-7-) methyltransferase|hcm1p|mRNA (guanine-7-)methyltransferase|mRNA (guanine-N(7)-)-methyltransferase|mRNA cap methyltransferase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RNMT, check out the RNMT Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RNMT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RNMT Antibody Picoband® (A07752-1)
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