Anti-RNMT Antibody Picoband®

Product Name

Anti-RNMT Antibody Picoband®

View all RNMT Antibodies

SKU/Catalog Number

A07752-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-RNMT Antibody Picoband® catalog # A07752-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-RNMT Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07752-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Standard Protein

E.coli-derived human RNMT recombinant protein (Position: R197-E380).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A07752-1 is reactive to RNMT in Human

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Observed Molecular Weight

56 kDa

Calculated molecular weight

49229 MW

Background of RNMT

Enables RNA binding activity and mRNA (guanine-N7-)-methyltransferase activity. Involved in 7-methylguanosine mRNA capping. Located in fibrillar center and nucleoplasm. Part of mRNA cap binding activity complex; mRNA cap methyltransferase complex; and receptor complex.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A07752-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Positive Control

WB: human Hela whole cell, human U2OS whole cell, human MOLT-4 whole cell
FCM: Daudi cell

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of RNMT using anti-RNMT antibody (A07752-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human U2OS whole cell lysates,
Lane 3: human MOLT-4 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNMT antigen affinity purified polyclonal antibody (Catalog # A07752-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNMT at approximately 56 kDa. The expected band size for RNMT is at 55 kDa.

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Figure 2. Flow Cytometry analysis of Daudi cells using anti-RNMT antibody (A07752-1).
Overlay histogram showing Daudi cells stained with A07752-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RNMT Antibody (A07752-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Gene/Protein Information For RNMT (Source: Uniprot.org, NCBI)

Alternative Names

DKFZp686H1252; EC 2.1.1.56; hcm1p; hCMT1; hCMT1c; hMet; KIAA0398; mRNA (guanine-7-)methyltransferase; mRNA (guanine-N(7)-)-methyltransferase; mRNA cap guanine-N7 methyltransferase; mRNA cap methyltransferase; RG7MT1MET; RNA (guanine-7-) methyltransferase RNMT CMT1, CMT1c, MET, Met, RG7MT1, cm1p, hCMT1, hMet RNA guanine-7 methyltransferase mRNA cap guanine-N7 methyltransferase|RNA (guanine-7-) methyltransferase|hcm1p|mRNA (guanine-7-)methyltransferase|mRNA (guanine-N(7)-)-methyltransferase|mRNA cap methyltransferase

For more info on RNMT, check out the RNMT Infographic

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In this infographic, you will see the following information for RNMT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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