Anti-RNF25 Antibody

Product Name

Anti-RNF25 Antibody

View all Rnf25 Antibodies

SKU/Catalog Number

A10595

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-RNF25 Antibody (Catalog # A10595). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-RNF25 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10595)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the carboxyl terminus of human RNF25 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A10595 is reactive to Rnf25 in Human, Mouse

Applications

A10595 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

51219 MW

Background of Rnf25

This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. RING finger protein 25 (RNF25, also named AO7) contains a RING finger domain and is ubiquitously expressed in various tissues. RNF25 was initially identified in a yeast two-hybrid screen of a murine T-cell library by using UbcH5b, an E2 enzyme, as bait. RNF25 has also been shown to act as a putative E3 ligase, at least in vitro. RNF25 localizes predominantly in the nucleus and supports the transcriptional activity of NF-kB by interacting with p65 in vivo upon stimulation with TNF. Yeast two-hybrid data also suggest that RNF25 interacts with a number of other molecules which may be potential ubiquitin ligase substrates. Among these are molecules that have critical roles in signal transduction and in regulation of translation (personal communication, Allan Weissman, CCR-NCI, Bethesda, MD).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:10,000 - 1:40,000
IP: 1 mg
WB: 1:200 - 1:2,000

Validation Images & Assay Conditions

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Western blot analysis of RNF25 expression in immunoprecipitation of 20

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Western blot analysis of RNF25 expression in HEK293 cells over-expressing human RNF25 (lane 1) and mouse RNF25 (lane 2). RNF25 was detected using rabbit anti-RNF25 Antigen Affinity purified polyclonal antibody (Catalog # A10595) at 1:1000. Personal Communication, Allan Weissman, CCR-NCI, Bethesda, MD.

Gene/Protein Information For Rnf25 (Source: Uniprot.org, NCBI)

Alternative Names

AO7; EC 6.3.2.-; FLJ13906; ring finger protein 25E3 ubiquitin-protein ligase RNF25; ring finger protein AO7

For more info on Rnf25, check out the Rnf25 Infographic

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In this infographic, you will see the following information for Rnf25: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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