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Product Info Summary
| SKU: | A15259-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-RNF186 Antibody Picoband®
SKU/Catalog Number
A15259-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-RNF186 Antibody Picoband® catalog # A15259-1. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RNF186 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15259-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human RNF186 recombinant protein (Position: R86-L159).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A15259-1 is reactive to RNF186 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
28 kDa
Calculated molecular weight
24145 MW
Background of RNF186
RNF186 is an E3 ubiquitin ligase that regulates endoplasmic reticulum (ER) stress and apoptosis through its interaction with BNIP1. This gene is mapped to chromosome 1p36.13 based on an alignment of theRNF186 sequence with the genomic sequence (GRCh38). Cotransfection and immunoprecipitation analyses showed that RNF186, through it transmembrane domains, interacted with BNIP1. Overexpression of RNF186 promoted BNIP1 ubiquitination and transfer of BNIP1 to mitochondria.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A15259-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human SW620 cell, human HepG2 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of RNF186 using anti-RNF186 antibody (A15259-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human SW620 cell lysates,
Lane 2: human HepG2 cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNF186 antigen affinity purified polyclonal antibody (Catalog # A15259-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNF186 at approximately 28KD. The expected band size for RNF186 is at 24KD.
Protein Target Info & Infographic
Gene/Protein Information For RNF186 (Source: Uniprot.org, NCBI)
Gene Name
RNF186
Full Name
E3 ubiquitin-protein ligase RNF186
Weight
24145 MW
Alternative Names
RING finger protein 186;RNF186; RNF186 ring finger protein 186 E3 ubiquitin-protein ligase RNF186
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RNF186, check out the RNF186 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RNF186: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RNF186 Antibody Picoband® (A15259-1)
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-RNF186 Antibody Picoband®
Question
Will anti-RNF186 antibody A15259-1 work on canine WB with colon mucosa?
Verified Customer
Verified customer
Asked: 2020-04-17
Answer
Our lab technicians have not validated anti-RNF186 antibody A15259-1 on canine. You can run a BLAST between canine and the immunogen sequence of anti-RNF186 antibody A15259-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine colon mucosa in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-17
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for kidney pancreas using anti-RNF186 antibody A15259-1. Let me know if you need anything else.
B. Baker
Verified customer
Asked: 2020-02-07
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-07
Question
Is this A15259-1 anti-RNF186 antibody reactive to the isotypes of RNF186?
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
The immunogen of A15259-1 anti-RNF186 antibody is E. coli-derived human RNF186 recombinant protein (Position: R86-L159). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-16
Question
We are currently using anti-RNF186 antibody A15259-1 for human tissue, and we are satisfied with the ELISA results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on dog tissues as well?
E. Baker
Verified customer
Asked: 2013-05-06
Answer
The anti-RNF186 antibody (A15259-1) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-05-06
