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Product Info Summary
| SKU: | A30667 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, IF (paraffin) |
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Product info
Product Name
Anti-RNF149 Antibody
SKU/Catalog Number
A30667
Size
100ul
Form
Liquid
Description
Boster Bio Anti-RNF149 Antibody (Catalog# A30667). Tested in IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RNF149 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30667)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human RNF149. Immunogen sequence location: 211-260
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A30667 is reactive to RNF149 in Human, Mouse
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
43165 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30667 is guaranteed for ELISA, IHC-P, IF (paraffin) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:10000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using RNF149 Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For RNF149 (Source: Uniprot.org, NCBI)
Gene Name
RNF149
Full Name
E3 ubiquitin-protein ligase RNF149
Weight
43165 MW
Alternative Names
RNF149;DNAPTP2;E3 ubiquitin-protein ligase RNF149;DNA polymerase-transactivated protein 2;RING finger protein 149
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RNF149, check out the RNF149 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RNF149: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RNF149 Antibody (A30667)
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Customer Q&As
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4 Customer Q&As for Anti-RNF149 Antibody
Question
Can you help my question with product A30667, anti-RNF149 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30667 anti-RNF149 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-13
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using anti-RNF149 antibody A30667. Let me know if you need anything else.
D. Dhar
Verified customer
Asked: 2019-11-07
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-07
Question
We are currently using anti-RNF149 antibody A30667 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-07
Answer
The anti-RNF149 antibody (A30667) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-07
Question
Will A30667 anti-RNF149 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-10-31
Answer
As indicated on the product datasheet, A30667 anti-RNF149 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-10-31
