Anti-RNF111 Antibody Picoband™

Product Name

Anti-RNF111 Antibody Picoband™

View all RNF111 Antibodies

SKU/Catalog Number

A05708-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-RNF111 Antibody Picoband™ catalog # A05708-1. Tested in WB, IHC, ICC/IF, FCM, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-RNF111 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05708-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Immunogen

E.coli-derived human RNF111 recombinant protein (Position: F134-S994).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A05708-1 is reactive to RNF111 in Human, Mouse, Rat

Applications

A05708-1 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

Background of RNF111

E3 ubiquitin-protein ligase Arkadia is an enzyme that in humans is encoded by the RNF111 gene. The protein encoded by this gene is a nuclear RING-domain containing E3 ubiquitin ligase. This protein interacts with the transforming growth factor (TGF) -beta/NODAL signaling pathway by promoting the ubiquitination and proteosomal degradation of negative regulators, like SMAD proteins, and thereby enhances TGF-beta target-gene transcription. As a modulator of the nodal signaling cascade, this gene plays a critical role in the induction of mesoderm during embryonic development. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Mouse
Immunohistochemistry, 2-5 μg/ml, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

There are currently no images for this product. We are working on uploading them please check back shortly or contact us to expedite our upload process for this antibody.

Gene/Protein Information For RNF111 (Source: Uniprot.org, NCBI)

Alternative Names

ARK; Arkadia; DKFZp313E0731; DKFZp761D081; E3 ubiquitin-protein ligase Arkadia; EC 6.3.2; EC 6.3.2.-; FLJ38008; ring finger protein 111DKFZp686H1966 RNF111 ARK, hRNF111 ring finger protein 111 E3 ubiquitin-protein ligase Arkadia|Arkadia|RING-type E3 ubiquitin transferase Arkadia

For more info on RNF111, check out the RNF111 Infographic

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In this infographic, you will see the following information for RNF111: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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