Anti-RIOK3 Antibody Picoband™

Product Name

Anti-RIOK3 Antibody Picoband™

View all RIOK3 Antibodies

SKU/Catalog Number

A09262-3

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-RIOK3 Antibody Picoband™ catalog # A09262-3. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-RIOK3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09262-3)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human RIOK3 recombinant protein (Position: Q48-E519).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A09262-3 is reactive to RIOK3 in Human

Applications

A09262-3 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

65 kDa

Calculated molecular weight

128790 MW

Background of RIOK3

This gene was first identified by the similarity of its product to the Aspergillus nidulans SUDD protein. This gene is now recognized as a member of the right open reading frame (RIO) kinase gene family. This gene encodes a serine/threonine kinase that localizes to the cytoplasm and plays a role in the processing of the pre-40 S ribosomal subunit. Alternative splicing results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of RIOK3 using anti-RIOK3 antibody (A09262-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RIOK3 antigen affinity purified polyclonal antibody (Catalog # A09262-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RIOK3 at approximately 65 kDa. The expected band size for RIOK3 is at 59 kDa.

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Figure 2. Flow Cytometry analysis of HepG2 cells using anti-RIOK3 antibody (A09262-3).
Overlay histogram showing HepG2 cells stained with A09262-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RIOK3 Antibody (A09262-3, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Gene/Protein Information For RIOK3 (Source: Uniprot.org, NCBI)

Alternative Names

DKFZp779L1370; EC 2.7.11.1; homolog of the Aspergillus nidulans sudD gene product; RIO kinase 3 (yeast); RIO kinase 3; serine/threonine-protein kinase RIO3; sudD (suppressor of bimD6, Aspergillus nidulans) homolog; sudD homolog; sudD suppressor of bimD6 homolog; SUDDsudD suppressor of Aspergillus nidulans bimD6 homolog RIOK3 SUDD RIO kinase 3 serine/threonine-protein kinase RIO3|homolog of the Aspergillus nidulans sudD gene product|sudD (suppressor of bimD6, Aspergillus nidulans) homolog|sudD homolog|sudD suppressor of Aspergillus nidulans bimD6 homolog|sudD suppressor of bimD6 homolog|testicular secretory protein Li 47

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