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Product Info Summary
| SKU: | A14138-2 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-RIMKLB Antibody Picoband®
SKU/Catalog Number
A14138-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-RIMKLB Antibody Picoband® catalog # A14138-2. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-RIMKLB Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14138-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human RIMKLB recombinant protein (Position: E25-D386).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A14138-2 is reactive to RIMKLB in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
48 kDa
Calculated molecular weight
64099 MW
Background of RIMKLB
Predicted to enable N-acetyl-L-aspartate-L-glutamate ligase activity and citrate-L-glutamate ligase activity. Predicted to be involved in glutamine family amino acid metabolic process. Predicted to be located in cytosol. Predicted to be active in cytoplasm.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14138-2 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Hela whole cell, human U20S whole cell
ICC/IF: Hela cell
FCM: HEL cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of RIMKLB using anti-RIMKLB antibody (A14138-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human U20S whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RIMKLB antigen affinity purified polyclonal antibody (Catalog # A14138-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RIMKLB at approximately 48 kDa. The expected band size for RIMKLB is at 42 kDa.
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Figure 2. IF analysis of RIMKLB and Tubulin beta using anti-RIMKLB antibody (A14138-2) and anti-Tubulin beta antibody (M05613-4).
RIMKLB and Tubulin beta was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RIMKLB Antibody (A14138-2) and mouse anti-Tubulin beta Antibody (M05613-4) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. Flow Cytometry analysis of HEL cells using anti-RIMKLB antibody (A14138-2).
Overlay histogram showing HEL cells stained with A14138-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RIMKLB Antibody (A14138-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For RIMKLB (Source: Uniprot.org, NCBI)
Gene Name
RIMKLB
Full Name
Beta-citrylglutamate synthase B
Weight
64099 MW
Superfamily
RimK family
Alternative Names
RNA-binding protein 47;RNA-binding motif protein 47;RBM47; RIMKLB FAM80B, NAAGS, NAAGS-I ribosomal modification protein rimK like family member B beta-citrylglutamate synthase B|N-acetyl-aspartyl-glutamate synthetase B|N-acetyl-aspartylglutamate synthetase B|N-acetylaspartyl-glutamate synthetase B|NAAG synthetase B|beta-citryl-glutamate synthase B|family with sequence similarity 80, member B|ribosomal protein S6 modification-like protein B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RIMKLB, check out the RIMKLB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RIMKLB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RIMKLB Antibody Picoband® (A14138-2)
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