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Product Info Summary
| SKU: | A30552 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, ICC |
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Product info
Product Name
Anti-RHG07 DLC1 Antibody
SKU/Catalog Number
A30552
Size
100ul
Form
Liquid
Description
Boster Bio Anti-RHG07 DLC1 Antibody (Catalog# A30552). Tested in IHC-P, IF, ICC, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RHG07 DLC1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30552)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human RHG07. Immunogen sequence location: 61-110
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A30552 is reactive to DLC1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
170591 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30552 is guaranteed for ELISA, IF, IHC-P, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry, 1:100 - 1:300
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:10000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human prostate carcinoma tissue, using RHG07 Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunofluorescence analysis of A549 cells, using RHG07 Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For DLC1 (Source: Uniprot.org, NCBI)
Gene Name
DLC1
Full Name
Rho GTPase-activating protein 7
Weight
170591 MW
Alternative Names
DLC1;ARHGAP7;KIAA1723;STARD12;Rho GTPase-activating protein 7;Deleted in liver cancer 1 protein;DLC-1;HP protein;Rho-type GTPase-activating protein 7;START domain-containing protein 12;StARD12;StAR-related lipid transfer protein DLC1 ARHGAP7, HP, STARD12, p122-RhoGAP DLC1 Rho GTPase activating protein rho GTPase-activating protein 7|Rho-GTPase-activating protein 7|START domain-containing protein 12|StAR-related lipid transfer (START) domain containing 12|deleted in liver cancer 1 protein|deleted in liver cancer 1 variant 2|deleted in liver cancer variant 4|epididymis secretory sperm binding protein|rho-type GTPase-activating protein 7
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DLC1, check out the DLC1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DLC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RHG07 DLC1 Antibody (A30552)
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No publications found for A30552
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Customer Q&As
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3 Customer Q&As for Anti-RHG07 DLC1 Antibody
Question
I have a question about product A30552, anti-RHG07 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-20
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30552 anti-RHG07 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-20
Question
Would anti-RHG07 antibody A30552 work on goat IHC with smooth muscle?
Verified Customer
Verified customer
Asked: 2018-01-19
Answer
Our lab technicians have not tested anti-RHG07 antibody A30552 on goat. You can run a BLAST between goat and the immunogen sequence of anti-RHG07 antibody A30552 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat smooth muscle in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-01-19
Question
We are currently using anti-RHG07 antibody A30552 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on canine tissues as well?
C. Yang
Verified customer
Asked: 2017-02-28
Answer
The anti-RHG07 antibody (A30552) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-02-28
