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Product Info Summary
| SKU: | M01299 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-Retinoid X Receptor alpha/RXRA Antibody Picoband® (monoclonal, 5E7)
View all RXR alpha/NR2B1 Antibodies
SKU/Catalog Number
M01299
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Retinoid X Receptor alpha/RXRA Antibody Picoband® (monoclonal, 5E7) catalog # M01299. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Retinoid X Receptor alpha/RXRA Antibody Picoband® (monoclonal, 5E7) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01299)
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Monoclonal
Clone Number
5E7
Isotype
Mouse IgG1
Immunogen
E. coli-derived human RXRA recombinant protein (Position: A226-T462).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M01299 is reactive to RXRA in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
55 kDa
Calculated molecular weight
50.811kDa
Background of RXR alpha/NR2B1
Retinoid X receptor alpha (RXR-alpha), also known as NR2B1 (nuclear receptor subfamily 2, group B, member 1) is a nuclear receptor that in humans is encoded by the RXRA gene. Retinoid X receptors (RXRs) and retinoic acid receptors (RARs) are nuclear receptors that mediate the biological effects of retinoids by their involvement in retinoic acid-mediated gene activation. These receptors function as transcription factors by binding as homodimers or heterodimers to specific sequences in the promoters of target genes. The protein encoded by this gene is a member of the steroid and thyroid hormone receptor superfamily of transcriptional regulators. Alternative splicing of this gene results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01299 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: human Hela whole cell,, human A549 whole cell,, human HepG2 whole cell,, human Caco-2 whole cell,, human HL-60 whole cell,, rat liver tissue,, mouse liver tissue
FCM: A549 cell, CACO-2 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Retinoid X Receptor alpha/RXRA using anti-Retinoid X Receptor alpha/RXRA antibody (M01299).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates;
Lane 2: human A549 whole cell lysates;
Lane 3: human HepG2 whole cell lysates;
Lane 4: human Caco-2 whole cell lysates;
Lane 5: human HL-60 whole cell lysates;
Lane 6: rat liver tissue lysates;
Lane 7: mouse liver tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Retinoid X Receptor alpha/RXRA antigen affinity purified monoclonal antibody (Catalog # M01299) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Retinoid X Receptor alpha/RXRA at approximately 55KD. The expected band size for Retinoid X Receptor alpha/RXRA is at 51KD.
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Figure 2. Flow Cytometry analysis of A549 cells using anti- Retinoid X Receptor alpha/RXRA antibody (M01299).
Overlay histogram showing A549 cells stained with M01299 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- Retinoid X Receptor alpha/RXRA Antibody (M01299, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Figure 3. Flow Cytometry analysis of CACO-2 cells using anti- Retinoid X Receptor alpha/RXRA antibody (M01299).
Overlay histogram showing CACO-2 cells stained with M01299 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- Retinoid X Receptor alpha/RXRA Antibody (M01299, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For RXRA (Source: Uniprot.org, NCBI)
Gene Name
RXRA
Full Name
Retinoic acid receptor RXR-alpha
Weight
50.811kDa
Superfamily
nuclear hormone receptor family
Alternative Names
Retinoic acid receptor RXR-alpha; Nuclear receptor subfamily 2 group B member 1; Retinoid X receptor alpha; RXRA; NR2B1 RXRA NR2B1 retinoid X receptor alpha retinoic acid receptor RXR-alpha|nuclear receptor subfamily 2 group B member 1|retinoid X nuclear receptor alpha
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RXRA, check out the RXRA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RXRA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Retinoid X Receptor alpha/RXRA Antibody Picoband® (monoclonal, 5E7) (M01299)
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