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Product Info Summary
| SKU: | A11219-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-REM2 Antibody Picoband®
SKU/Catalog Number
A11219-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-REM2 Antibody Picoband® catalog # A11219-2. Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-REM2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11219-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human REM2 recombinant protein (Position: R65-E257). Human REM2 shares 94.8% and 95.9% amino acid (aa) sequence identity with mouse and rat REM2, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A11219-2 is reactive to REM2 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Calculated molecular weight
37.275kDa
Background of Rem2
Predicted to enable GTP binding activity and calcium channel regulator activity. Predicted to be involved in negative regulation of high voltage-gated calcium channel activity. Predicted to be active in plasma membrane.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11219-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of REM2 using anti-REM2 antibody (A11219-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human SH-SY5Y whole cell lysates,
Lane 3: human Hacat whole cell lysates,
Lane 4: human Hela whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-REM2 antigen affinity purified polyclonal antibody (A11219-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for REM2 at approximately 37 kDa. The expected band size for REM2 is at 37 kDa.
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Figure 2. Flow Cytometry analysis of Jurkat cells using anti-REM2 antibody (A11219-2).
Overlay histogram showing Jurkat cells stained with A11219-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-REM2 Antibody (A11219-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For REM2 (Source: Uniprot.org, NCBI)
Gene Name
REM2
Full Name
GTP-binding protein REM 2
Weight
37.275kDa
Superfamily
small GTPase superfamily
Alternative Names
CD300LE; FLJ38964; GTP-binding protein REM 2; ICLM2; Rad and Gem-like GTP-binding protein 2; Rad and Gem-related 2 (rat homolog); RAS (RAD and GEM) like GTP binding 2; RAS (RAD and GEM)-like GTP binding 2 Rem2|RRAD and GEM like GTPase 2|GTP-binding protein REM 2|GEM, REM, Kir family small G-protein|GTP-binding protein REM2|RAS (RAD and GEM) like GTP binding 2|Ras-related GTP-binding protein of the Rad/Gem/Kir family, member 2|rad and Gem-like GTP-binding protein 2|rad and gem related GTP binding protein 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on REM2, check out the REM2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for REM2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-REM2 Antibody Picoband® (A11219-2)
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