Anti-RBMX Antibody (Center)

Product Name

Anti-RBMX Antibody (Center)

View all hnRNP G Antibodies

SKU/Catalog Number

M04738

Size

80 µl

Description

Boster Bio Anti-RBMX Antibody (Center) (Catalog # M04738). Tested in WB, IHC-P application(s). This antibody reacts with Human.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-RBMX Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04738)

Host

Rabbit

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

This RBMX antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 262-294 amino acids from the Central region of human RBMX.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M04738 is reactive to Rbmx in Human

Applications

M04738 is guaranteed for IHC-P, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

42332 Da

Background of hnRNP G

RNA-binding protein that plays several role in the regulation of pre- and post-transcriptional processes. Implicated in tissue-specific regulation of gene transcription and alternative splicing of several pre-mRNAs. Binds to and stimulates transcription from the tumor suppressor TXNIP gene promoter; may thus be involved in tumor suppression. When associated with SAFB, binds to and stimulates transcription from the SREBF1 promoter. Associates with nascent mRNAs transcribed by RNA polymerase II. Component of the supraspliceosome complex that regulates pre-mRNA alternative splice site selection. Can either activate or suppress exon inclusion; acts additively with TRA2B to promote exon 7 inclusion of the survival motor neuron SMN2. Represses the splicing of MAPT/Tau exon 10. Binds preferentially to single- stranded 5'-CC[A/C]-rich RNA sequence motifs localized in a single-stranded conformation; probably binds RNA as a homodimer. Binds non-specifically to pre-mRNAs. Plays also a role in the cytoplasmic TNFR1 trafficking pathways; promotes both the IL-1- beta-mediated inducible proteolytic cleavage of TNFR1 ectodomains and the release of TNFR1 exosome-like vesicles to the extracellular compartment.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000-1:2000
IHC-P: 1:25

Validation Images & Assay Conditions

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All lanes : Anti-RBMX Antibody (Center) at 1:1000-1:2000 dilution
Lane 1: Hela whole cell lysates
Lane 2: K562 whole cell lysates
Lane 3: Jurkat whole cell lysates
Lane 4: A431 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 42 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

Click image to see more details

M04738 staining RBMX in human testis tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Gene/Protein Information For Rbmx (Source: Uniprot.org, NCBI)

Alternative Names

Glycoprotein p43; heterogeneous nuclear ribonucleoprotein G; hnRNP G; hnRNP-G; HNRPG; RBMXP1; RBMXRT; RNA binding motif protein, X chromosome; RNA binding motif protein, X-linked; RNA-binding motif protein, X chromosome; RNMX Rbmx|hnRNP G|RNA binding motif protein, X chromosome|RNA-binding motif protein, X chromosome|heterogeneous nuclear ribonucleoprotein G

For more info on Rbmx, check out the Rbmx Infographic

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In this infographic, you will see the following information for Rbmx: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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