Anti-RBCK1 (UBCE7IP3) Antibody

Product Name

Anti-RBCK1 (UBCE7IP3) Antibody

View all RBCK1 Antibodies

SKU/Catalog Number

M04562

Size

50 µl

Description

Boster Bio Anti-RBCK1 (UBCE7IP3) Antibody (Catalog # M04562). Tested in WB application(s). This antibody reacts with Human.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-RBCK1 (UBCE7IP3) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04562)

Host

Mouse

Contents

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Monoclonal

Clone Number

1839CT718.19.54

Isotype

IgG1,k

Immunogen

This RBCK1 (UBCE7IP3) antibody is generated from a mouse immunized with a recombinant protein of human RBCK1 (UBCE7IP3).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M04562 is reactive to RBCK1 in Human

Applications

M04562 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

57572 Da

Background of RBCK1

E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, such as UBE2L3/UBCM4, and then transfers it to substrates. Functions as an E3 ligase for oxidized IREB2 and both heme and oxygen are necessary for IREB2 ubiquitination. Promotes ubiquitination of TAB2 and IRF3 and their degradation by the proteasome. Component of the LUBAC complex which conjugates linear ('Met-1'-linked) polyubiquitin chains to substrates and plays a key role in NF- kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF- RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Together with FAM105B/otulin, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis. Binds polyubiquitin of different linkage types.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:2000

Validation Images & Assay Conditions

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All lanes : Anti-RBCK1 (UBCE7IP3) Antibody at 1:2000 dilution
Lane 1: Jurkat whole cell lysate
Lane 2: 293 whole cell lysate
Lane 3: A375 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 58 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

Gene/Protein Information For RBCK1 (Source: Uniprot.org, NCBI)

Alternative Names

C20orf18; EC 6.3.2.-; HBV associated factor 4; HBV-associated factor 4; Heme-oxidized IRP2 ubiquitin ligase 1; Hepatitis B virus X-associated protein 4; HOIL-1; RanBP-type and C3HC4-type zinc finger containing 1; ranBP-type and C3HC4-type zinc finger-containing protein 1; RBCC protein interacting with PKC1; RBCK2; RNF54RING finger protein 54; UBCE7IP3HOIL1; ubiquitin conjugating enzyme 7 interacting protein 3; Ubiquitin-conjugating enzyme 7-interacting protein 3; XAP3; XAP4chromosome 20 open reading frame 18; ZRANB4 RBCK1 C20orf18, HOIL-1, HOIL1, PBMEI, PGBM1, RBCK2, RNF54, UBCE7IP3, XAP3, XAP4, ZRANB4 RANBP2-type and C3HC4-type zinc finger containing 1 ranBP-type and C3HC4-type zinc finger-containing protein 1|HBV-associated factor 4|RBCC protein interacting with PKC1|RING finger protein 54|RING-type E3 ubiquitin transferase HOIL-1|heme-oxidized IRP2 ubiquitin ligase 1|hepatitis B virus X-associated protein 4|ubiquitin conjugating enzyme 7 interacting protein 3

For more info on RBCK1, check out the RBCK1 Infographic

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In this infographic, you will see the following information for RBCK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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