Anti-RANTES/CCL5 Antibody Picoband®

Product Name

Anti-RANTES/CCL5 Antibody Picoband®

View all CCL5/RANTES Antibodies

SKU/Catalog Number

PB9573

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-RANTES/CCL5 Antibody Picoband® catalog # PB9573. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-RANTES/CCL5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9573)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human RANTES recombinant protein (Position: Y26-S91). Human RANTES shares 83.3% amino acid (aa) sequence identity with both mouse and rat RANTES.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9573 is reactive to CCL5 in Human

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

11 kDa

Calculated molecular weight

9990 MW

Background of CCL5/RANTES

Chemokine (C-C motif) ligand 5 (also CCL5) is a protein which in humans is encoded by the CCL5 gene. It is also known as RANTES. This gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CC subfamily, functions as a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor chemokine (C-C motif) receptor 5 (CCR5), and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Alternative splicing results in multiple transcript variants that encode different isoforms.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PB9573 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, -
ELISA, 0.1-0.5μg/ml, -

Positive Control

WB: HUT Whole Cell

Validation Images & Assay Conditions

Gene/Protein Information For CCL5 (Source: Uniprot.org, NCBI)

Alternative Names

C-C motif chemokine 5;EoCP;Eosinophil chemotactic cytokine;SIS-delta;Small-inducible cytokine A5;T cell-specific protein P228;TCP228;T-cell-specific protein RANTES;RANTES (3-68);RANTES (4-68);CCL5;D17S136E, SCYA5; CCL5 D17S136E, RANTES, SCYA5, SIS-delta, SISd, TCP228, eoCP C-C motif chemokine ligand 5 C-C motif chemokine 5|T-cell specific protein p288|beta-chemokine RANTES|chemokine (C-C motif) ligand 5|eosinophil chemotactic cytokine|regulated upon activation, normally T-expressed, and presumably secreted|small inducible cytokine subfamily A (Cys-Cys), member 5

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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