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Product Info Summary
| SKU: | A04648-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-RAMP/ZMYM2 Antibody Picoband®
SKU/Catalog Number
A04648-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-RAMP/ZMYM2 Antibody Picoband® catalog # A04648-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-RAMP/ZMYM2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04648-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human RAMP/ZMYM2 recombinant protein (Position: K573-R1281).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04648-1 is reactive to ZMYM2 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
150 kDa
Calculated molecular weight
154.911kDa
Background of ZNF198
Zinc finger MYM-type protein 2 is a protein that in humans is encoded by the ZMYM2 gene. The protein encoded by this gene is a zinc finger protein that may act as a transcription factor. The encoded protein may be part of a BHC histone deacetylase complex. Translocation of this gene with the fibroblast growth factor receptor-1 gene (FGFR1) results in a fusion gene, which may be a cause of stem cell leukemia lymphoma syndrome (SCLL). Several transcript variants encoding the same protein have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04648-1 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.5-1 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human MOLT-4 whole cell, rat C6 whole cell, mouse EL-4 whole cell
ICC/IF: U20S cell
FCM: HL-60 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of RAMP/ZMYM2 using anti-RAMP/ZMYM2 antibody (A04648-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MOLT-4 whole cell lysates,
Lane 2: rat C6 whole cell lysates,
Lane 3: mouse EL-4 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAMP/ZMYM2 antigen affinity purified polyclonal antibody (Catalog # A04648-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAMP/ZMYM2 at approximately 150 kDa. The expected band size for RAMP/ZMYM2 is at 155 kDa.
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Figure 2. IF analysis of RAMP/ZMYM2 using anti-RAMP/ZMYM2 antibody (A04648-1).
RAMP/ZMYM2 was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RAMP/ZMYM2 Antibody (A04648-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. Flow Cytometry analysis of HL-60 cells using anti-RAMP/ZMYM2 antibody (A04648-1).
Overlay histogram showing HL-60 cells stained with A04648-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAMP/ZMYM2 Antibody (A04648-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For ZMYM2 (Source: Uniprot.org, NCBI)
Gene Name
ZMYM2
Full Name
Zinc finger MYM-type protein 2
Weight
154.911kDa
Alternative Names
Solute carrier family 12 member 5; Electroneutral potassium-chloride cotransporter 2; K-Cl cotransporter 2; hKCC2; Neuronal K-Cl cotransporter; SLC12A5; KCC2; KIAA1176 ZMYM2 FIM, MYM, RAMP, SCLL, ZNF198 zinc finger MYM-type containing 2 zinc finger MYM-type protein 2|fused in myeloproliferative disorders protein|rearranged in an atypical myeloproliferative disorder|zinc finger protein 198|zinc finger, MYM-type 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ZMYM2, check out the ZMYM2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ZMYM2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RAMP/ZMYM2 Antibody Picoband® (A04648-1)
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