V(D)J recombination-activating protein 1 (RAG1)

Catalytic component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. From the RAG complex, RAG1 mediates the DNA-binding into the conserved recombination signal sequences (RSS) and catalyzes the DNA cleavage activities by introducing a double-strand break between the RSS and the adjacent coding section.

RAG2 isn't a catalytic component but is required for all known catalytic activities. DNA cleavage occurs in two steps: a first nick is introduced at the top strand immediately upstream of the heptamer, generating a 3'- hydroxyl group that may attack the phosphodiester bond on the reverse strand in a direct transesterification reaction, thereby creating 4 DNA endings: 2 hairpin coding ends and two blunt, 5'- phosphorylated ends.