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Product Info Summary
| SKU: | A04172 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, IF (paraffin) |
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Product info
Product Name
Anti-RABEP1/Rabaptin 5 Antibody
SKU/Catalog Number
A04172
Size
100ul
Form
Liquid
Description
Boster Bio Anti-RABEP1/Rabaptin 5 Antibody (Catalog# A04172). Tested in IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RABEP1/Rabaptin 5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04172)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human RABEP1. Immunogen sequence location: 549 - 598
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A04172 is reactive to RABEP1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
72 kDa
Calculated molecular weight
99290 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04172 is guaranteed for ELISA, IHC-P, IF (paraffin) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:40000
Not yet tested in other applications.
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using RABEP1 Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For RABEP1 (Source: Uniprot.org, NCBI)
Gene Name
RABEP1
Full Name
Rab GTPase-binding effector protein 1
Weight
99290 MW
Superfamily
rabaptin family
Alternative Names
RABEP1;RAB5EP;RABPT5;RABPT5A;Rab GTPase-binding effector protein 1;Rabaptin-4;Rabaptin-5;Rabaptin-5alpha;Renal carcinoma antigen NY-REN-17 RABEP1 RAB5EP, RABPT5 rabaptin, RAB GTPase binding effector protein 1 rab GTPase-binding effector protein 1|neurocrescin|rabaptin-4|rabaptin-5|rabaptin-5alpha|renal carcinoma NY-REN-17
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RABEP1, check out the RABEP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RABEP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RABEP1/Rabaptin 5 Antibody (A04172)
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No publications found for A04172
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Customer Q&As
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13 Customer Q&As for Anti-RABEP1/Rabaptin 5 Antibody
Question
Is there a BSA free version of anti-RABEP1/Rabaptin 5 antibody A04172 available?
Verified Customer
Verified customer
Asked: 2020-04-06
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-RABEP1/Rabaptin 5 antibody A04172 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-06
Question
My question regarding product A04172, anti-RABEP1/Rabaptin 5 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-01
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04172 anti-RABEP1/Rabaptin 5 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-01
Question
We want to test anti-RABEP1/Rabaptin 5 antibody A04172 on human cervix carcinoma for research purposes, then I may be interested in using anti-RABEP1/Rabaptin 5 antibody A04172 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
V. Zhao
Verified customer
Asked: 2019-09-10
Answer
The products we sell, including anti-RABEP1/Rabaptin 5 antibody A04172, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-10
Question
Would anti-RABEP1/Rabaptin 5 antibody A04172 work on monkey IHC with liver?
G. Anderson
Verified customer
Asked: 2019-08-12
Answer
Our lab technicians have not validated anti-RABEP1/Rabaptin 5 antibody A04172 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-RABEP1/Rabaptin 5 antibody A04172 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey liver in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-12
Question
Here is the WB image, lot number and protocol we used for cervix carcinoma using anti-RABEP1/Rabaptin 5 antibody A04172. Please let me know if you require anything else.
D. Jha
Verified customer
Asked: 2019-08-08
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-08
Question
I see that the anti-RABEP1/Rabaptin 5 antibody A04172 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-04-11
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-04-11
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-RABEP1/Rabaptin 5 antibody A04172. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-09-12
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-09-12
Question
Does anti-RABEP1/Rabaptin 5 antibody A04172 work for IHC with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2018-08-30
Answer
According to the expression profile of cervix carcinoma, RABEP1 is highly expressed in cervix carcinoma. So, it is likely that anti-RABEP1/Rabaptin 5 antibody A04172 will work for IHC with cervix carcinoma.
Boster Scientific Support
Answered: 2018-08-30
Question
I was wanting to use your anti-RABEP1/Rabaptin 5 antibody for IHC for human cervix carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human cervix carcinoma identification?
Verified Customer
Verified customer
Asked: 2018-08-27
Answer
It shows on the product datasheet, A04172 anti-RABEP1/Rabaptin 5 antibody has been tested for IHC on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-08-27
Question
Is this A04172 anti-RABEP1/Rabaptin 5 antibody reactive to the isotypes of RABEP1?
P. Parker
Verified customer
Asked: 2017-04-24
Answer
The immunogen of A04172 anti-RABEP1/Rabaptin 5 antibody is A peptide derived from human RABEP1. Immunogen sequence location: 549 - 598. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-04-24
Question
We are currently using anti-RABEP1/Rabaptin 5 antibody A04172 for rat tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?
T. Huang
Verified customer
Asked: 2014-12-09
Answer
The anti-RABEP1/Rabaptin 5 antibody (A04172) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-12-09
Question
Does A04172 anti-RABEP1/Rabaptin 5 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
B. Carter
Verified customer
Asked: 2014-07-15
Answer
As indicated on the product datasheet, A04172 anti-RABEP1/Rabaptin 5 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-07-15
Question
Is a blocking peptide available for product anti-RABEP1/Rabaptin 5 antibody (A04172)?
M. Mitchell
Verified customer
Asked: 2013-01-25
Answer
We do provide the blocking peptide for product anti-RABEP1/Rabaptin 5 antibody (A04172). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-01-25
