Product Info Summary
SKU: | M05148-1 |
---|---|
Size: | 100 μl/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-RAB5C / RABL Rabbit Monoclonal Antibody
SKU/Catalog Number
M05148-1
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-RAB5C / RABL Rabbit Monoclonal Antibody catalog # M05148-1. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RAB5C / RABL Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05148-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
28R55
Isotype
IgG
Immunogen
A synthesized peptide derived from human RAB5C / RABL
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M05148-1 is reactive to RAB5C in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
23 kDa
Calculated molecular weight
27680 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05148-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
WB: human 293T whole cell, human HepG2 whole cell, human A549 whole cell, human RT4 whole cell, rat RH35 whole cell, mouse HEPA1-6 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of RAB5C/RABL using anti-RAB5C/RABL antibody (M05148-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human RT4 whole cell lysates,
Lane 5: rat RH35 whole cell lysates,
Lane 6: mouse HEPA1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB5C/RABL antigen affinity purified monoclonal antibody (Catalog # M05148-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAB5C/RABL at approximately 23 kDa. The expected band size for RAB5C/RABL is at 23 kDa.
Protein Target Info & Infographic
Gene/Protein Information For RAB5C (Source: Uniprot.org, NCBI)
Gene Name
RAB5C
Full Name
Ras-related protein Rab-5C
Weight
27680 MW
Superfamily
small GTPase superfamily
Alternative Names
Caspase-14;CASP-14;3.4.22.-;Caspase-14 subunit p17, mature form;Caspase-14 subunit p10, mature form;Caspase-14 subunit p20, intermediate form;Caspase-14 subunit p8, intermediate form;CASP14; RAB5C L1880L, RAB5L, RABL, RAB5C RAB5C, member RAS oncogene family ras-related protein Rab-5C|RAB5C, member of RAS oncogene family
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RAB5C, check out the RAB5C Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RAB5C: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RAB5C / RABL Rabbit Monoclonal Antibody (M05148-1)
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