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Product Info Summary
| SKU: | PB9791 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Rab18 Antibody Picoband®
SKU/Catalog Number
PB9791
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Rab18 Antibody Picoband® catalog # PB9791. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Rab18 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9791)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Rab18, identical to the related mouse sequence, and different from the related rat sequence by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9791 is reactive to RAB18 in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
23 kDa
Calculated molecular weight
22977 MW
Background of Rab18
Ras-related protein Rab-18 is a protein that in humans is encoded by the RAB18 gene. It is a ubiquitously expressed protein with particularly high expression in the brain. The protein encoded by this gene is a member of a family of Ras-related small GTPases that regulate membrane trafficking in organelles and transport vesicles. Knockdown studies in zebrafish suggest that this protein may have a role in eye and brain development. Mutations in this gene are associated with Warburg micro syndrome type 3. Alternatively spliced transcript variants have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9791 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: Rat Testis Tissue, Rat Lung Tissue, 293T Whole Cell, HELA Whole Cell, HEPG2 Whole Cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Rab18 using anti-Rab18 antibody (PB9791).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Rat Testis Tissue Lysate at 50ug,
Lane 2: Rat Lung Tissue Lysate at 50ug,
Lane 3: 293T Whole Cell Lysate at 40ug,
Lane 4: HELA Whole Cell Lysate at 40ug,
Lane 5: HEPG2 Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Rab18 antigen affinity purified polyclonal antibody (Catalog # PB9791) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Rab18 at approximately 23 kDa. The expected band size for Rab18 is at 23 kDa.
Protein Target Info & Infographic
Gene/Protein Information For RAB18 (Source: Uniprot.org, NCBI)
Gene Name
RAB18
Full Name
Ras-related protein Rab-18
Weight
22977 MW
Superfamily
small GTPase superfamily
Alternative Names
Ras-related protein Rab-18;RAB18; Rab18|AA959686|RAB18, member RAS oncogene family|ras-related protein Rab-18|GTP-binding protein Rab18
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RAB18, check out the RAB18 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RAB18: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Rab18 Antibody Picoband® (PB9791)
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Customer Q&As
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1 Customer Q&As for Anti-Rab18 Antibody Picoband®
Question
We are currently using anti-Rab18 antibody PB9791 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, rat. Is it possible that the antibody can work on canine tissues as well?
S. Williams
Verified customer
Asked: 2013-12-06
Answer
The anti-Rab18 antibody (PB9791) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-12-06
