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Product Info Summary
| SKU: | PB9788 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-RAB10 Antibody Picoband®
SKU/Catalog Number
PB9788
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-RAB10 Antibody Picoband® catalog # PB9788. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RAB10 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9788)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human RAB10, identical to the related mouse sequence, and different from the related rat sequence by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9788 is reactive to RAB10 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
23 kDa
Calculated molecular weight
22541 MW
Background of RAB10
Ras-related protein Rab-10 is a protein that in humans is encoded by the RAB10 gene. RAB10 belongs to the RAS superfamily of small GTPases. And RAB proteins localize to exocytic and endocytic compartments and regulate intracellular vesicle trafficking. The RAB10 gene is mapped to chromosome 2p23.1-p22.3 by radiation hybrid analysis. It was found that the purified recombinant GAP domain of human AS160 showed GAP activity with RAB2A, RAB8A, RAB10, and RAB14, but not with 14 other RABs. Immunoblot analysis showed that these RABs associated with Glut4-positive vesicles in mouse adipocytes. Thereby, it concluded that AK160 functions as a RAB GAP and that RABs may participate in GLUT4 translocation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9788 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: human MCF-7 whole cell, human Hela whole cell, human A549 whole cell, rat brain tissue, rat C6 whole cell, mouse brain tissue, mouse NIH/3T3 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of RAB10 using anti-RAB10 antibody (PB9788).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: mouse brain tissue lysates,
Lane 7: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB10 antigen affinity purified polyclonal antibody (Catalog # PB9788) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAB10 at approximately 23 kDa. The expected band size for RAB10 is at 23 kDa.
Protein Target Info & Infographic
Gene/Protein Information For RAB10 (Source: Uniprot.org, NCBI)
Gene Name
RAB10
Full Name
Ras-related protein Rab-10
Weight
22541 MW
Superfamily
small GTPase superfamily
Alternative Names
Ras-related protein Rab-10;RAB10; RAB10 RAB10, member RAS oncogene family ras-related protein Rab-10|GTP-binding protein RAB10|ras-related GTP-binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RAB10, check out the RAB10 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RAB10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RAB10 Antibody Picoband® (PB9788)
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1 Customer Q&As for Anti-RAB10 Antibody Picoband®
Question
We are currently using anti-RAB10 antibody PB9788 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-20
Answer
The anti-RAB10 antibody (PB9788) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-20
