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Product Info Summary
| SKU: | M13434 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Dog, Human, Monkey, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, WB |
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Product info
Product Name
Anti-PUS7 Mouse Monoclonal Antibody [Clone ID: OTI4C6]
SKU/Catalog Number
M13434
Size
100 µl
Description
Boster Bio PUS7 mouse monoclonal antibody, clone OTI4C6 (formerly 4C6). Catalog# M13434. Tested in FC, IF, WB. This antibody reacts with Human, Monkey, Mouse, Rat, Dog.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-PUS7 Mouse Monoclonal Antibody [Clone ID: OTI4C6] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M13434)
Host
Mouse
Contents
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI4C6
Isotype
IgG1
Immunogen
Full length human recombinant protein of human PUS7 (NP_061915) produced in HEK293T cell.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M13434 is reactive to PUS7 in Dog, Human, Monkey, Mouse, Rat
Reconstitution
Calculated molecular weight
75.035kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M13434 is guaranteed for Flow Cytometry, IF, WB Boster Guarantee
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PUS7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PUS7.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PUS7 monoclonal antibody.
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Flow cytometric Analysis of Hela cells
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Flow cytometric Analysis of Jurkat cells
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Anti-PUS7 mouse monoclonal antibody (M13434) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PUS7.
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Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT) and PUS7-Knockout HeLa cells (KO) were separated by SDS-PAGE and immunoblotted with anti-PUS7 monoclonal antibody M13434 (1:1000`). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.
Protein Target Info & Infographic
Gene/Protein Information For PUS7 (Source: Uniprot.org, NCBI)
Gene Name
PUS7
Full Name
Pseudouridylate synthase 7 homolog
Weight
75.035kDa
Superfamily
pseudouridine synthase TruD family
Alternative Names
EC 5.4.99.-; FLJ20485; KIAA1897; MGC17720; pseudouridylate synthase 7 homolog (S. cerevisiae); pseudouridylate synthase 7 homolog PUS7 IDDABS pseudouridine synthase 7 pseudouridylate synthase 7 homolog|pseudouridylate synthase 7 (putative)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PUS7, check out the PUS7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PUS7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PUS7 Mouse Monoclonal Antibody [Clone ID: OTI4C6] (M13434)
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No publications found for M13434
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